大孔树脂吸附分速溶茶副产品中咖啡碱、茶多酚的研究.pdfVIP

摘要 在速溶茶的工业生产中，常见的工艺是使用超滤膜过滤茶提取液，过滤 中会产生一定量的截留液，将这些截留液进行转溶再过滤，再次被截留的溶 液经浓缩干燥后即得到速溶茶副产品，这部分产品依然含有茶叶有效成分，如 茶多酚、咖啡碱。如果能将速溶茶副产品充分的回收再利用不仅能给企业带来一 定的经济效益还可以避免对环境造成污染。 在转溶过程中会添加酸或碱进行转溶，这就使速溶茶副产品中带有一部分 盐，在进行大孔树脂吸附前先对这部分产品进行脱盐处理。以电导率、咖啡碱和 茶多酚的回收率为考察指标，选择较好的脱盐树脂。 本文选用六种大孔树脂对速溶茶副产品溶液中咖啡碱、茶多酚进行静态吸附 试验，以咖啡碱为主要检测指标、茶多酚为次要检测指标考察各种树脂的吸附性 能。从中筛选出D101 树脂具有较好的静态吸附效果，然后对该树脂进行动态试 验。在动态吸附试验中设计了上样流速、样液浓度和样液温度的单因素试验，在 单因素试验的基础上进行三因素三水平的正交试验，确定了较佳的吸附工艺参 数：上样流速 1.5mL/min，上样液温度 60 ℃，咖啡碱上样浓度 0.08mg/mL ， 茶多酚上样浓度8.0mg/mL；确定吸附条件之后进行动态解析试验，通过对洗 脱流速、洗脱剂浓度、洗脱剂用量的单因素试验和正交优化试验，确定了较 佳的解析条件：洗脱流速为 1.0mL/min、洗脱剂乙醇体积分数为 80%、洗脱 剂用量为4BV 。以此优化条件进行树脂分离，咖啡碱含量从6%提高到23% ， 茶多酚含量从38.03%提高到60.87% ，得率达到16.85%。 关键词：速溶茶副产品，大孔树脂，咖啡碱，茶多酚，吸附，解析 I Abstract In the commercial production of instant tea, the ultrafiltration is commonly used in extraction of tea. And during this filter process, a certain amount of retention fluid can be produced. In the next step ,the retention fluid were re-dissolved and re-filtered, similarly retention fluid produced. And instant tea by-product is to be obtained by drying the retention fluid of the second filter step. The by-product still contains some active ingredients of tea, such as tea poly-phenols, caffeine. Recycling these active ingredients from instant tea by-product is not only benefit to company ，but also prevents pollution to the environment. Alkali is usually used in re-dissolving process, as a result ，a certain of salt generated. Thus desalination is needed before Macroporous resin adsorption. The conductivity, the recovery of caffeine and tea polyphenols are considered to select the better desalting resin. In this paper, six products macroporous resins were used to adsorb staticly