可逆识别Hg2+的罗丹明基荧光增强型探针及在活细胞成像中的应用有机化学.pdfVIP

DOI: 10.6023/cjoc201305013 研究论文 有机化学 Chinese Journal of Organic Chemistry ARTICLE 可逆识别Hg2＋的罗丹明基荧光增强型探针及在活细胞成像中的应用 陈稼轩 田 怡* 向清祥 熊俊如 张丽群 李 艳 (乐山师范学院化学学院 乐山 614000) 摘要 基于罗丹明类荧光团, 合成了一个新型的 Hg2＋荧光探针 RS. 通过紫外光谱法和荧光光谱法研究了探针 RS 在 EtOH-HEPES 缓冲液( V ∶V＝1 ∶1, pH＝7.3)中对 Hg2＋的识别特性. 结果表明: 探针 RS 可快速、可逆识别 Hg2＋. 随着 2＋ 2＋ Hg 的滴加, 探针 RS 的内酰胺环结构发生开环, 并形成 1 ∶1 Hg 配合物. 在 L929 活细胞中的荧光显微成像表明 RS 可应用于生物体的检测. 关键词 罗丹明 B; 荧光探针; 可逆性; Hg2＋离子; 活细胞 A Reversible Fluorescence Enhancement Probe for Hg2＋ Based on Rhodamine and Its Application in Live Cell Imaging Chen, Jiaxuan Tian, Yi* Xiang, Qingxiang Xiong, Junru Zhang, Liqun Li, Yan (Department of Chemistry, Leshan Normal University, Leshan 614000) Abstract A novel of Hg2＋ fluorescence probe (RS) with rhodamine chromophore was synthesized, and its structure was characterized by 1H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg2＋ ions had been investigated in EtOH-HEPES buffer solution ( V ∶V＝1 ∶1, pH＝7.3) by the ultraviolet spectrophotometry and the fluo- rescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg2＋, the spirolactam ring of probe RS was opened and 1 ∶1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg2＋ in L929 living cells demonstrated that the probe RS could be used in biological systems. Keywords rhodamine B; fluorescent probe; reversibility; H