Genetic Steps to Organ Laterality in Zebrafish.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2001; 2: 60–68. DOI: 10.1002/cfg.74 Research Article Genetic steps to organ laterality in zebrafish 1 2 1 1 1 Jau-Nian Chen , Frauke van Bebber , Allan M. Goldstein , Fabrizio C. Serluca , Donald Jackson , 1 1 1 1 1 1 Sarah Childs , George Serbedzija , Kerri S. Warren , John D. Mably , Per Lindahl , Alan Mayer , 2 1 Pascal Haffter and Mark C. Fishman * 1Cardiovascular Research Center, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA 2 Max-Planck-Institut fu¨r Entwicklungsbiologie, Abteilung Genetik, Spemannstrasse 35, 72076, Tu¨bingen, Germany Abstract *Correspondence to: M. C. Fishman, Cardiovascular All internal organs are asymmetric along the left–right axis. Here we report a genetic Research Center, Massachusetts screen to discover mutations which perturb organ laterality. Our particular focus is upon General Hospital, 149 13th whether, and how, organs are linked to each other as they achieve their laterally Street, Charlestown, MA 02129, asymmetric positions. We generated mutations by ENU mutagenesis and examined F3 USA and Department of Medicine, Harvard Medical progeny using a cocktail of probes that reveal early primordia of heart, gut, liver and School, Boston, MA 02115, USA. pancreas. From the 750 genomes examined, we isolated seven recessive