不同镉积累型苋菜（Amaranthus mangostanus L.）根际低分子量有机酸与镉吸收的关系.pdfVIP

中国农业科学 2007,40(12):2727-2733 Scientia Agricultura Sinica 不同镉积累型苋菜（Amaranthus mangostanus L.） 根际低分子量有机酸与镉吸收的关系 范洪黎，王 旭，周 卫 （中国农业科学院农业资源与农业区划研究所/农业部植物营养与养分循环重点开放实验室，北京 100081 ） 摘要：【目的】研究低镉积累型苋菜紫背苋和高镉积累型苋菜天星米在镉胁迫下低分子量有机酸分泌特征及 其与苋菜镉生物积累的关系。【方法】采用溶液培养法进行试验。【结果】随供镉浓度增加，紫背苋根、茎和叶生 物量显著降低，而天星米生物量未发生明显改变。不同镉暴露条件下，苋菜分泌的有机酸总量表现为天星米远高 于紫背苋，有机酸含量均表现为柠檬酸苹果酸乙酸丙酸丁酸，且以柠檬酸和苹果酸为主。两品种各有机酸数 量占有机酸总量的比例基本一致。随供镉浓度增加，两个苋菜品种根分泌的各有机酸量增加，各有机酸分泌量的 增幅均表现为天星米大大高于紫背苋，植株根、茎和叶镉含量与积累量也相应表现为天星米大大高于紫背苋。【结 论】苋菜镉的生物积累与有机酸种类没有特异性关系，但与有机酸的总量有关。镉胁迫诱导根系分泌有机酸的数 量，在引起两品种对镉吸收的差异方面起重要作用。 关键词：苋菜；镉；低分子量有机酸 Low Molecular Weight Organic Acids in Rhizosphere and Their Effects on Cadmium Accumulation in Two Cultivars of Amaranth (Amaranthus mangostanus L.) FAN Hong-li, WANG Xu, ZHOU Wei (Key Laboratory of Plant Nutrition and Nutrient Cycling of the Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081) Abstract: 【Objective 】 To examine whether low- molecular weight organic acids (LMWOAs) in root exudates of amaranth play a role in Cd hyperaccumulation, the amounts and composition of LMWOAs isolated from two cultivars of amaranth (Amaranthus mangostanus L.) ，Zibeixian, a low Cd accumulator, and Tianxingmi, a high Cd accumulator were compared. 【Method 】 Plants were grown hydroponically and three treatments (Cd 0, 1 and 2.5 mg·L-1) were conducted for 1 wk before isolation of LMWOAs. 【Result 】 With the increase of solution Cd concentration, the biomass of Zibeixian decreased dramatically, while those of Tianxingmi remained stable. In all treatments, total amount of LMWOAs in rhizosp