菜薹-芥蓝单体异附加系n+1配子传递及两个二体异附加系的获得.pdfVIP

中国农业科学 2010,43(23):4871-4876 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.23.012 菜薹-芥蓝单体异附加系n+1配子传递 及两个二体异附加系的获得 陈雪平，申二巧，张成合，李晓峰，轩淑欣，申书兴 （河北农业大学园艺学院，河北保定 071001 ） 摘要：【目的】研究菜薹-芥蓝单体异附加系n+1配子传递，为其在基因定位和遗传改良上的应用奠定基础。 【方法】通过观察和统计各单体异附加系减数分裂后期Ⅱ10/10/11/11 分离的 PMCs，估算 n+1 配子的形成频率； 通过鉴定回交子代植株的染色体数目，测定各单体异附加系 n+1 配子的传递率；从单体异附加系的自交子代中筛 选二体异附加系。【结果】依据减数分裂后期Ⅱ10/10/11/11 分离的 PMCs 比率估算，各单体异附加系 n+1 雄配子 的形成频率在 36.85%—45.15%；基于染色体数目鉴定，各单体异附加系 n+1 雄配子的传递率在 7.14%—14.81%， 雌配子在 19.70%—36.51%；在一定范围内，n+1 配子传递率与 n+1 配子形成频率、染色体大小、花粉量和花粉生 活力没有显著的相关性；从自交子代中获得了两个不同的二体异附加系。【结论】菜薹-芥蓝各单体异附加系的额 外染色体通过雌、雄配子均能进行传递，但传递率因不同的附加系而异；从单体异附加系的自交子代中可以分离 出遗传性稳定的二体异附加系。 关键词：菜薹；芥蓝；附加系；n+1配子形成和传递 Transmission of n+1 Gametes and Obtaining of Two Bisomic Addition Lines of Flowering Chinese Cabbage-Chinese Kale CHEN Xue-ping, SHEN Er-qiao, ZHANG Cheng-he ，LI Xiao-feng, XUAN Shu-xin, SHEN Shu-xing (College of Horticulture, Agricultural University of Hebei, Baoding 071001, Hebei) Abstract: 【Objective 】 Transmission of the n+1 gametes of flowering Chinese cabbage-Chinese kale monosomic alien addition lines was studied in order to lay a basis for application of the gene localization and genetic improvement. 【Method 】 The frequencies of n+1 gamete formation in different monosomic addition lines were assessed by a rate of PMCs with 10/10/11/11 segregation at anaphase Ⅱ. The transmission rates were determined by examining the chromosome number of plants derived from the populations of the different monosomic addition lines crossed with the flowering Chinese cabbage, respectively. The bis