地表覆膜对柑橘果实糖积累及蔗糖代谢酶活性的影响.pdfVIP

中国农业科学 2013,46(2):317-324 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.02.011 地表覆膜对柑橘果实糖积累及蔗糖代谢酶活性的影响 姜 妮，高海文，金龙飞，刘永忠，彭抒昂 （华中农业大学园艺林学学院/ 园艺植物生物学教育部重点实验室，武汉430070 ） 摘要：【目的】研究地表覆膜（plastic film mulching，PFM）促进柑橘果实糖分积累增加的调控机制，丰 富柑橘果实优质品质的形成理论知识。【方法】以鄂柑 1 号椪柑为材料,比较柑橘果实成熟期覆膜和对照之间 叶片中可溶性总糖和淀粉含量、果实糖分积累以及蔗糖相关代谢酶活性的差异。【结果】与对照相比,覆 膜处理使土壤水势降低,最终叶片中的可溶性总糖含量、果肉囊瓣皮和汁胞中可溶性糖含量显著增高；处 理中囊瓣皮中的酸性转化酶和蔗糖合酶分解活性比对照显著增加,而汁胞中蔗糖合酶的分解活性降低,合 成活性增加。【结论】覆膜使土壤产生了一定的水分胁迫，水分亏缺胁迫下诱发的渗透调节机制，使果实 中糖分含量增加。其中蔗糖合酶是在覆膜情况下提高果肉库强，促进果实糖分积累增加的关键酶。 关键词：椪柑；覆膜；糖积累；蔗糖代谢酶 The Impact of Plastic Film Mulching on Citrus Fruit Sugar Accumulation and Sucrose Metabolism-Related Enzymes JIANG Ni, GAO Hai-wen, JIN Long-fei, LIU Yong-zhong, PENG Shu-ang (College of Horticulture and Forestry Sciences, Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070) Abstract: 【Objective 】The aim of this research is to investigate the possible physiological mechanism of the effect of plastic film mulching on citrus fruit sugar accumulation, which could help to enrich the knowledge of citrus high fruit quality formation. 【Method 】 ‘Egan 1’ Ponkan (Citrus reticulata cv. Egan 1) was used as material, sugar accumulation and activities of sucrose metabolism-related enzymes were compared in fruit segment membrane and juice sacs, as well as contents of leaf total soluble sugar and starch between the plastic film mulching treatments and the control. 【Result 】 Plastic film mulching at fruit ripening stage reduced soil water potential, which in the end increased the total soluble sugar content in leaf, fruit segment membrane and juice sacs. On the other hand, the activities of ac