动物源大肠杆菌PMQR基因流行性检测.pdfVIP

中国农业科学 2012,45(10):2052-2057 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.10.018 动物源大肠杆菌PMQR 基因流行性检测 庄 娜，陈雪影，岳 磊，廖晓萍，刘雅红 （华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室，广州 510642 ） 摘要：【目的】检测分离自广东省散养型养殖场的动物源大肠杆菌中 oqxAB 基因及其它质粒介导喹诺酮类耐 药基因(PMQR)的流行情况。 【方法】采用PCR 方法检测oqxA、oqxB、qnr、qepA 和aac （6′）-Ib-cr 基因；琼脂 平板稀释法对PMQR 阳性菌株进行18 种抗菌药物的最小抑菌浓度测定。 【结果】qnrB 、qnrS、aac （6′）-Ib-cr、 oqxA、oqxB 的检出率依次为10.49%、18.88%、31.47%、44.8%和48.9%，但未检测到qnrA、qnrC、qnrD 和qepA。 oqxA 与oqxB 的检出率较高，且oqxA 与oqxB 常常同时存在。多数菌株同时携带两个或两个以上的PMQR 基因。PMQR 阳性菌对18 种兽医临床常用抗生素耐药率较高。【结论】PMQR 基因在广东省兽医临床传播广泛，广东省大肠杆菌 对临床常用抗菌药物耐药较为严重，药敏谱呈多样化，多重耐药株比例较高。 关键词：oqxAB 基因；大肠埃希氏菌；质粒介导；喹诺酮；耐药基因 Detection of PMQR Gene in Escherichia coli Isolated from Animals ZHUANG Na, CHEN Xue-ying, YUE Lei, LIAO Xiao-ping, LIU Ya-hong (Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation /College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642) Abstract: 【Objective 】The objective of the study is to investigate the prevalence of oqxAB gene and other three types of plasmid-mediated quinolone resistance ( PMQR) gene in Escherichia coli isolated from free-range farms in Guangdong province. 【Method 】All the strains were screened for the oqxA, oqxB, qnr, qepA, aac (6′)-Ib-cr genes by PCR. The susceptibility of PMQR- positive strains to 18 antimicrobial agents were determined by agar dilution method. 【Results 】The positive rates of qnrB, qnrS, aac （6′）-Ib-cr，oqxA，oqxB genes were 10.49%, 18.88%, 31.47%, 44.8%, and 48.9% respectively. No qnrA, qnrC, qnrD and qepA genes were detected in any of the isolates. oqxA and oqxB have a high positive rate, and the two genes always exsist in the same isolates. PMQR gene always sh