甘蓝型油菜杂交种亲本的遗传多样性评价.pdfVIP

中国农业科学 2004,37(11):1766-1771 Scientia Agricultura Sinica 甘蓝型油菜杂交种亲本的遗传多样性评价 周国岭 刘平武 杨光圣 傅廷栋 华中农业大学作物遗传改良国家重点实验室/ 国家油菜改良武汉分中心 武汉 430070 摘要 利用SSR和ISSR分子标记对18份不育系 21份恢复系和11份对照品种共50份甘蓝型油菜进行了遗 传多样性评价 UPGMA聚类结果显示 50份材料被聚为四大类群 其中所有的不育系聚在第四大类 恢复系都聚 在了其它三大类中 不育系又分为2个亚类 其中核不育系聚为一亚类(除1471AB外) 而所有的细胞质雄性不育 系均聚为另一亚类 不育系间的遗传差异比恢复系间小 关键词 甘蓝型油菜 杂交种亲本 遗传多样性 SSR; ISSR Evaluation of the Genetic Diversity of Hybrid Parents in Brassic napus ZHOU Guo-ling, LIU Ping-wu, YANG Guang-sheng, FU Ting-dong (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University/ Research Center of National Rapeseed Improvement, Wuhan 430070) Abstract: Genetic diversity of 50 Brassic napus varities including 18 sterile lines, 21 restorer lines and 11 check cultivars was estimated by SSR and ISSR. The UPGMA results showed that 50 Brassica napus varities were divided into 4 groups. The restorer lines tested were classified into Group I-III respectively; The sterile lines tested were classified into Group IV. In addition, The sterile lines could be further divided into two subgroups: except 1471AB, all genic male sterile lines were in subgroup I, 1471AB and all cytoplasmic male sterile lines were in subgroup II. The UPGMA results also indicated that the restorer lines had more genetic diversity than the sterile lines. Key words: Brassic napus; Hybrid parents; Genetic diversity; SSR; ISSR 雄性不育是油菜杂种优势利用的一条重要途径 用SSR 标记评价其遗传多样性并进行品种鉴定和纯度 在杂种优势利用中亲本选配是配制强优势组合 有效 分析成为可能 利用油菜杂种优势的关键环节 全面 系统地了解油 本研究结合SSR 标记和ISSR 标记对50 份甘蓝型 菜杂交种亲本的亲缘关系 系谱来源 遗传多样性及 油菜材料进行遗传多样性评价 系统地分析了材料间 材料间遗传距离