杉木应压木木质部细胞形态特征及主要代谢成分表征.pdfVIP

第37卷摇 第5期 北 京 林 业 大 学 学 报 Vol.37,No.5 2015年5月 JOURNAL OF BEIJING FORESTRY UNIVERSITY May,2015 鄄鄄 DOI:10.13332/ j.1000 1522.座机电话号码 杉木应压木木质部细胞形态特征及主要代谢成分表征 张胜龙摇 刘京晶摇 楼雄珍摇 刘摇 洋摇 童再康摇 黄华宏 浙江农林大学亚热带森林培育国家重点实验室培育基地 摘要:为探讨主要代谢成分与应压木形成的关系,以拉弯诱导形成的杉木应压木为材料,在明确其重要显微特征、 木质素含量的基础上,运用气相色谱质谱联用系统 GC鄄MS 对主要代谢成分进行了鉴定和相对表达丰度分析。 结 果表明:杉木应压木管胞多以椭圆形呈现,且管胞壁明显增厚。 应压木管胞平均长度和宽度分别为 1347郾34 和 20郾18滋m,均显著小于对应木;平均壁腔比为0郾43,显著大于对应木。 应压木木质素的相对含量较对应木增加 21郾9%。 在3个拉弯时期皆可鉴定出18种代谢成分,其中有机酸5种、单糖4种、二糖3种、醇类和氨基酸各2种、 无机酸和内酯类各1种。 时期 玉应压木中果糖和葡萄糖含量与对应木差异不大,但这2种化合物含量均随拉弯时 间延长呈现下降趋势,且时期域、芋在应压木中的含量显著小于对应木,符合应压木纤维素含量下降的事实。 应压 木中与木质素合成相关的莽草酸含量呈上升趋势,其在时期芋应压木中的含量显著高于对应木,这解释了应压木 中木质素含量明显多于对应木的现象。 关键词:杉木;应压木;结构特征;代谢成分;气相色谱质谱联用系统 鄄鄄 鄄鄄 鄄鄄 中图分类号:S78郾3摇 摇 文献标志码:A摇 摇 文章编号:1000 1522 2015 05 0126 08 ZHANG Sheng鄄long;LIUJing鄄jing;LOUXiong鄄zhen;LIUYang;TONGZai鄄kang;HUANGHua鄄hong. Morphological characteristics of cells and main metabolic components in xylem of Cunninghamia 鄄鄄 lanceolata compression wood. Journal of Beijing Forestry University 2015 37 5 126 133 [Ch,37 ref.]Nurturing Station for the State Key Laboratory of Subtropical Forest, Zhejiang Agriculture and Forestry University,Lin爷an,Zhejiang,311300,P.R. China. To explore the relationship between major metabolic components and the formation of compression wood CW ,we took the Cunninghamia lanceolata CW induced by bending as the study material,and then clarifiedthe anatomic characteristicsandlignin contentsof C. lanceolata CW. Furtherweused GC鄄 MS to indentify the major metabolic components from the developed xylem and analyzed the relative abundance. The results showed that the most of CW tracheids were oval and developed with thick cell walls. Average length and width of CW tracheidswere 1347郾34 滋m and20郾18 滋m respectively,which were significantly less than those of opposite wood OW . The average ratio of cell wall to lumen was 0郾43,obviously higher thanthat of OW. Therelative content of CW increasedby21郾9% comparedwith the OW. Eighteen known metabolites and iso