欧美山杨杂种插穗不定根2个发育时期基因表达谱分析.pdfVIP

第37卷摇 第10期 北 京 林 业 大 学 学 报 Vol.37,No.10 2015年10月 JOURNAL OF BEIJING FORESTRY UNIVERSITY Oct. ,2015 鄄鄄 DOI:10.13332/ j.1000 1522.座机电话号码 欧美山杨杂种插穗不定根2个发育时期基因表达谱分析 1 1 1 2 1 闫绍鹏 摇 尚艳茹摇 冷淑娇 摇 杨瑞华摇 王秋玉 1东北林业大学生命科学学院摇 2 黑龙江省农业科学院园艺分院 摘要:以欧美杂种山杨嫩枝插穗为实验材料,进行扦插不定根生成过程中插穗形态和解剖学观察,通过Solexa高通 量测序对扦插后6和12d插穗不定根生成部位进行转录水平上的检测分析。 插穗解剖观察发现扦插后6d为根原 基的诱导期,12d为不定根的伸长生长期,对插穗不定根2个时期高通量测序获得差异表达基因2328个。 对筛选 出的显著差异表达基因进行功能注释、分类和KEGG及pathway分析,确定2个山杨样本中差异表达基因参与的生 化代谢和信号转导途径。 关键词:杂种山杨;基因表达谱;扦插;差异表达 鄄鄄 鄄鄄 鄄鄄 中图分类号:S718郾43摇 摇 文献标志码:A摇 摇 文章编号:1000 1522 2015 10 0009 05 1 1 1 2 1 YAN Shao鄄peng ; SHANG Yan鄄ru ; LENG Shu鄄jiao ; YANG Rui鄄hua ; WANG Qiu鄄yu . Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root. 鄄鄄 Journal of Beijing Forestry University 2015 37 10 9 13 [Ch,13 ref.] 1 Collegeof Life Sciences,Northeast Forestry University,Harbin,Heilongjiang,150040,P. R. China; 2 Horticultural Branch,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang,150069, P. R. China. We used Populus tremula 伊 P. tremuloides softwood cuttings as experimental material, and observed morphological and anatomical changes of the cuttings during the rooting process of adventitious roots. Solexa high鄄throughput sequencing was performed for the parts of cuttingswhere adventitiousroots generated 6 days and 12 days after cutting DAC at transcriptional level. Anatomical observation showed that the induction stage of the adventitious root primordia was 6 days after cutting and the elongation stage of adventitious roots was 12 days after cutting. A total of 2328 differentially expressed geneswere obtained by the Solexa high鄄throughput sequencing. The significantly differential expression geneswere classified after their functions annotation,and then the KEGG passway analysis was carried out. On this basis,the biochemical metabolic and signal transduction pathways of these differentially expressed geneswere determined. Key words摇 hybrid aspen;gene expression profi