全血谷胱甘肽还酶活性系数(BGRAC)最佳分析测定条件的探讨.pdfVIP

澎ALAW义华 川99.6 硬_L居戈 全血谷肮甘肚还原醉活性系数(BGRAC) 最佳分析侧定条件探讨 摘 要 本研究对影响全塑全Ktt,}还C酶字西I系ff((BGRAC)f析 测定的因素进行了较全面探讨，对顾景范改良法可进行以下改 进:①制溶血液时加抗凝剂EDTA;OONADH割弋NADPH作底物; ③用柠檬酸一柠檬酸钠缓冲溶液代替KH2P0,Na2HP0缓冲溶液;④ 加入FAD后预保温时间由5分钟改为2分钟:⑤显色剂DTNB用量 由0.30m1改为0.20ml;⑥用离心法酬弋过滤法去蛋白质沉淀。并对 所用生化试剂的稳定性进行了探讨。改进后的分析方法的线性范围为 0^-60.Opm/lL，相对标准偏差为1.74%^4.17%，回收率为84补, 94.1%,BGRAC测定结果与顾氏法比较无显著性差异 ((P0.20)。 本法具有操作简便、分析成本低、失渡误差较小、适合大批量样品分 析等优点，具有掬 应‘用价值。 关链词:BGRAC 反应条件\ 侧定条件 脚劝这护护人贷 19996 颐圣老灵 Abstract Allreaction conditionsand determination conditions for measuringbloodglutathionereductaseactivationcoefficientwere studied.InstudyGujingfansapproachwasimprovedinthe _ a氏， rollowmgaspects:.vtW IA usesmnemolysate;UNicotmamide adeninedinucleotidereduceddisodiumsaltreplac以Nicotinamide adeninedinucleotidephosphatereducedsodium saltusedas substrate;(sSodiumcitratebufferreplaces(potassiumphosphate buffer..Only2minutespreincubationofhemolysatewithFAD Wksnecessaryformaximalactivation;⑥Theprecipitationof proteinscanberemovedbycentrifugation.Thestabilityof biochemistryreagentswasalsostudied.Therelativestandard deviationandtherateofrecoverybytheimprovedmethodwere W as na 1.74%一4.17%and84.7%一94.1%respectively.There significantdifferencebetweentheresultsbytheimprovedmethod andtheresultsbyGujingfansmethod(P0.20).The method切ok less timeandlessmoneytodetermineBGRAC thanGujingfansdidTheimprovedmethodwasalsoverysimple andconvenient. 琴eywords:BGRAC reactionconditions determinationconditions 脚存西荞7人 }1999.6 硬 IiEX 全血谷脸甘肚还