动物内源性多肽CGA-N46对念珠菌细胞增殖的影响.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2014 2 动物 内源性多肽 CGA-N46 对念珠菌细胞增殖的影响 阎晓慧  李瑞芳  张慧茹  尹艳杰  卢研博  卢亚丽 （河南工业大学生物工程学院，郑州 450001） 摘 要 ： CGA-N46 是人嗜铬粒蛋白N 端具有抗真菌活性的多肽片段。采用MTT 法检测CGA-N46 对克柔念珠菌生长的抑制 作用，以β-1，3-葡聚糖酶活性检测克柔念珠菌细胞壁的完整性，采用PI 检测克柔念珠菌细胞膜通透性，用罗丹明123 检测线粒 体膜电位。试验结果证实，0.5 mg/mL 的CGA-N46 能够明显抑制克柔念珠菌的细胞增殖能力；对其细胞壁的完整性及细胞膜的通 透性没有影响 ；CGA-N46 能明显降低线粒体膜电位。推测CGA-N46 可能通过影响线粒体膜电位来抑制克柔念珠菌的生长。 关键词 ： CGA-N46 克柔念珠菌 抑菌作用 线粒体膜电位 - Effect of Animal Endogenous Polypeptide CGA N46 on Cell Proliferation of Candidas Yan Xiaohui Li Ruifang Zhang Huiru Yin Yanjie Lu Yanbo Lu Yali （College of Bioengineering ，Henan University of Technology ，Zhengzhou 45000 1 ） Abstract:  CGA-N46 is an N terminal derived peptide of human Chromogranin A (CGA) that has anti-fungal activities. The growth inhibition effect on Candida krusei was measured with MTT assay. The cell wall integrity of Candida krusei was examined by monitoring β-1,3- glucanase activity. The effect of CGA-N46 on the permeability of Candida krusei membrane was examined using Propidium iodide detection. The effect of CGA-N46 on mitochondrial membrane potential was evaluated through Rh-123 assay. The results of showed 0.5 mg/mL CGA-N46 could significantly inhibit the proliferation of the C. krusei cells. CGA-N46 had no obvious effect on the β -1, 3-glucanase activity. There was no significant influence on the permeability of C. krusei cell membrane; CGA-N46 could significantly decrease the mitochondrial membrane potential. A