RNAi干扰interference.pptx

生物大分子结构与功能;上课安排：;RNA interference（RNAi） RNA干扰;RNA interference;RNA interference;Molecular Biology’s Central Dogma;TYPE OF RNA;Types of RNA;RNA interference, RNAiRNA干扰;What is RNA interference (RNAi)?;RNAi terms;Alternate terms to RNAi;The History of RNAi Discoveries;1990-Petunias(矮牵牛);Overexpression of chalcone synthase in petunias unexpectedly resulted in white petunias;Two mechanisms can explain this transgene-mediated gene silencing Transcriptional gene silencing Post Transcriptional Gene Silencing (PTGS) mRNA is made, but then degraded;1992-The mold(霉菌）;In 1995 Guo and Kemphues wanted to show that they had cloned the C. elegans par-1 gene (required for normal division of the zygote).Used antisense RNA to prove and sense RNA as control;Both the antisense and sense strands effectively silenced;‘Antisense’ Technology?;Craig Mello;Andrew Fire;Fire Mello hypothesize on how sense works?;1998-Fire et al and Mello;;1998-Fire et al. and Mello, Nature;;;Andrew Fire Craig Mello;Nobel Prize in Physiology or Medicine 2006;Essense of Nobel Prize;RNAi现象广泛存在于线虫，果蝇，斑马鱼，真菌以及植物等生物体内。 这些生物体利用RNAi来抵御病毒的感染，阻断转座子的作用。 RNAi能高效特异的阻断基因的表达，在线虫、果蝇体内，RNAi能达到基因敲除的效果。 在小鼠和人的体外培养细胞中利用RNAi技术也成功阻断了基因的表达，实现了细胞水平的基因敲除。 ;RNAi studies in PubMed;How does RNAi work?;siRNA biogenesis;Some applications of RNAi;Genome-wide Screens;Genome-wide RNAi;Systems Biology and RNAi;Networks of Early Embryogenesis;Systems Biology Approach:Three networks in one;RNAi Effects Examination;1999-Hamilton Baulcombe;;;;Summary;THANK YOU!