2型糖尿病伴轻度认知功能障碍患者血清生物标志物分析.docVIP

2型糖尿病伴轻度认知功能障碍患者血清生物标志物分析 【摘要】 目的：研究2型糖尿病伴轻度认知功能障碍患者外周血清生物标志物。方法：选取2013年8月-2015年8月在笔者所在医院就诊的67例2型糖尿病患者作为研究对象，根据认知功能评分量表将其分为认知功能正常组（32例，C组）和轻度认知功能障碍组（35例，M组）。采用酶联免疫吸附反应（ELISA）检测血清中脑源性神经营养因子（BDNF）、肿瘤坏死因子-α（TNF-α）、晚期糖基化终末产物（AGEs）及其受体（RAGE）等生物标志物的表达水平，并与认知功能量表评分进行相关性分析。结果：与C组相比，M组患者血清BDNF显著下调，且TNF-α、AGEs和RAGE表达均显著上调（P 0.05）。Pearson相关分析结果表明，BDNF、TNF-α、AGEs和RAGE与认知功能量表评分均有显著相关性（P 0.05）。结论：外周血清神经营养因子下调及炎症反应激活可能与2型糖尿病伴轻度认知功能障的发病机制有关。 【关键词】 2型糖尿病； 认知功能障碍； 脑源性神经营养因子； 炎症介质 中图分类号 R446 文献标识码 A 文章编号 1674-6805（2016）13-0008-03 【Abstract】 Objective：To investigate the serum biomarkers in patients with type 2 diabetes mellitus with mild cognitive impairment.Method：Sixty-seven patients with type 2 diabetes in our hospital from August 2013 to August 2015 were selected as the research objects.According to cognitive function rating scale，the patients were divided into the normal groups（32 cases，group C）and the mild cognitive impairment group（35 cases，group M）.Enzyme-linked Immune-Sorbent assay（ELISA） kits were used to measure the brain-derived neurotrophic factor（BDNF），tumor necrosis factors-α（TNF-α），advanced glycation end product （AGEs） and its receptor（RAGE），and the correlation analysis was made with the cognitive function scale score.Result：Patients in M group showed a significant decrease of BDNF，and a significant increase of TNF-α，AGEs and RAGE in serum than that of those in C group（P 0.05）.Pearson correlation analysis showed that serum BDNF，TNF-α，AGEs and RAGE levels were significantly correlated with scores of cognitive function（P 0.05）.Conclusion：The down-regulation of neurotrophic factor in peripheral serum and activation of inflammatory response may be related to the pathogenesis of type 2 diabetes mellitus with mild cognitive impairment. 【Key words】 Type 2 diabetes； Cognitive dysfunction； Brain derived neurotrophic factor； Inflammatory mediators First-author’s address：The Forth People’s Hospital of Taizhou，Taizhou 225300，China 糖尿病是临床常见疾病，据统计全世界约有5%～7%的人群罹患该病[1]。目前，糖尿病的治疗措施主要依赖于