B7―H3在神经胶质瘤免疫逃逸过程中的作用机制研究.docVIP

B7―H3在神经胶质瘤免疫逃逸过程中的作用机制研究 　　摘要：目的：通过对不同级别的神经胶质瘤（glioma）组织中共刺激分子B7-H3的检测，进一步探讨B7-H3在神经胶质瘤免疫逃逸过程中的作用机制，为神经胶质瘤临床免疫治疗提供理论基础和实验依据。方法：采用免疫组化法，检测58例神经胶质瘤患者瘤组织和9例正常脑组织中B7-H3的表达，并分析B7-H3的表达与神经胶质瘤生物学行为及愈后之间的关系。结果：58例不同级别的胶质瘤组织均表达B7-H3，9例正常脑组织中发现很少有B7-H3的表达，低级别（1～2级）及高级别（3～4级）胶质瘤组与正常脑组织组之间B7-H3的表达分别有差异性（P0.05）， 低级别（1～2级）胶质瘤组与高级别（3～4级）胶质瘤组之间有明显差异性（P0.05），B7-H3的表达与患者的年龄、性别无关，与病理级别及愈后有关，生存期超过2年的神经胶质瘤患者B7- H3 阳性表达率显著高于生存期短于2年的胶质瘤患者（ P?0.01）。结论：B7-H3的表达与神经胶质瘤具有相关性，随神经胶质瘤病理级别的增高而降低，在神经胶质瘤的免疫逃逸过程中有重要作用，可以作为反映神经胶质瘤生物学行为和患者预后的一个新的指标； 并为神经胶质瘤免疫逃逸机制的进一步研究提供了线索，为临床制定合理的治疗方案、预后判断及疗效评定提供客观参考依据。 　　关键词：B7-H3；神经胶质瘤；免疫逃逸 　　Abstract： Objective： Through exploring the expressions of costimulatory molecules B7-H3 with gliomas， To investigate the relationship between B7-H3 and the immunological escaping mechanism of gliomas，and offer theoretical and experimental evidence for studying gliomas.Methods： The protein expression of B7-H3 was examined by immunohistochemical method in 58 gliomas and 9 normal brains. The relationship between the expression levels of B7-H3 and the glioma biological behaviors was analyzed . Results： Expression of B7-H3 were found in 58 gliomas， very little in 9 normal brains. The expression level of B7-H3 reduced obviously accompany with the incrasing of the grade of gliomas（P0.05）. Each group was significantly different（P0.05）.B7-H3 expression was not related to age and sex， There was a close correlation between B7 - H3 expression and several factors， such as histologic types and prognosis （P 0.05） .The positive rate of B7 - H3 expression in patients more than two years survival were significantly higher than that in patients less than two years survival （P0.05）. Conclusions：There was a close correlation between B7-H3 and glioma ，the expression of B7-H3 reduced with the increase of grades of glioma， Detection of B7-H3 expression in gliomas will be beneficial to the judgment the biological behaviors and prognosis of gliomas .And be significance to evaluate the biologica