IL―27与TGF―β1在肺纤维化患者血清中的表达及意义.docVIP

IL―27与TGF―β1在肺纤维化患者血清中的表达及意义 　　[摘要] 目的 探讨IL-27和TGF-β1在肺纤维化患者血清中的表达及临床意义。 方法 采用ELISA法分别检测20例肺纤维化患者及15例健康对照组血清中的IL-27和TGF-β1的表达水平。 结果 （1）肺纤维化重度缺氧组均具有更高的IL-27与TGF-β1水平，并且不同缺氧程度的肺纤维化IL-27与TGF-β1水平均逐渐升高，从对照组、肺纤维化轻度缺氧组、中度缺氧组、重度缺氧组均不同，差异均有统计学意义（F=21.58，P0.001；F=18.75，P0.001）。（2）肺纤维化血清IL-27与TGF-β1水平均随PaO2升高而明显降低，经直线相关分析发现二者与PaO2均分别呈负相关（r=-0.754，P0.05；r=-0.793，P0.05）；血清IL-27和TGF-β1之间呈正相关（r=0.854，P0.05）。结论 IL-27和TGF-β1参与了肺纤维化发病，二者检测对其诊治有着重要的临床意义。 　　[关键词] IL-27；TGF-β1；肺纤维化 　　[中图分类号] R563.9 [文献标识码] A [文章编号] 1673-9701（2015）13-0009-03 　　[Abstract] Objective To investigate the expression and clinical significance of IL-27 and TGF-β1 in patients with pulmonary fibrosis. Methods The expression levels of IL-27 and LTB4 were determined by ELISA method in 20 cases of pulmonary fibrosis patients and 15 cases of healthy control group. Results （1） The levels of IL-27 and TGF-β1 in pulmonary fibrosis patients were higher than the control group；IL-27 and TGF-β1 levels were gradually increased from the control group， pulmonary fibrosis mild hypoxia group， moderate hypoxia group， severe hypoxia group， the differences were statistically significant （F=21.58， P0.001； F=18.75， P0.001）；（2）The serum IL-27 and TGF-β1 levels were significantly reduced with the increase of PaO2， the linear correlation was found between the two groups and PaO2 were negatively correlated（r=-0.754，P0.05； r=-0.793，P0.05）； It was positively correlated between IL-27 and TGF-β1（r=0.854，P0.05）. Conclusion IL-27 and TGF-β1 were being involved in the pathogenesis of pulmonary fibrosis， detection simultaneously， there were important significances for pulmonary fibrosis clinic diagnosis and therapy. 　　[Key words] IL-27； TGF-β1； Pulmonary fibrosis 　　肺间质纤维化是一种主要以成纤维细胞增殖、细胞外基质过度沉积等为病理特征的渐进性、致死性疾病， 其支气管肺泡上皮受损、肺组织结构形成不可逆性重塑，预后极差，但病因至今尚不完全明确，近年来研究表明多种细胞因子在肺间质纤维化的发病中起着重要作用，IL-27可以通过固有免疫系统和适应性免疫系统的各种细胞而发挥广泛的免疫调节，研究已表明，IL-27可通过调节Th1、Th2细胞及相关细胞因子参与感染性疾病；也可通过调节Th17、Th1、Th2细胞及其相关细胞因子参与自身免疫性疾病，IL-27的基因有望成为治疗肺纤维化的靶基因。TGF-β1目前已有研究证实与