两种方式提取杏鲍菇菌丝胞外酶的比较分析.docVIP

两种方式提取杏鲍菇菌丝胞外酶的比较分析 摘要：为了寻找有效提取杏鲍菇（Pleurotus eryngii Quel）菌丝胞外酶的方法，为杏鲍菇等食用菌胞外酶的活性研究和生长规律提供试验依据，研究通过pH 4.8的柠檬酸缓冲液、去离子水2种处理方式提取杏鲍菇菌丝胞外酶，并通过测定8种胞外酶酶活来对这2种方式进行比较分析。结果表明，2种提取方式对杏鲍菇菌丝胞外酶的酶活有差异，其中，酸性纤维素酶和果胶酶经酸处理酶活是水处理的2倍多;木聚糖酶和木质素降解相关酶类经酸处理的酶活也高于水处理，但淀粉酶和蛋白酶经酸处理酶活低于水处理。 关键词：杏鲍菇（Pleurotus eryngii）;胞外酶;酸处理;水处理;比较分析 中图分类号：S646.1+4 文献标识码：A 文章编号：0439-8114（2015）02-0427-04 DOI：10.14088/j.cnki.issn0439-8114.2015.02.043 Comparative Analyses of Two Methods of Extracting Extracellular Enzymes from Pleurotus eryngii LUO Wei，XIE Chun-liang，YAN Li，ZHU Zuo-hua，LI Zhi-min，HU Zhen-xiu，PENG Yuan-de （Institute of Bast Fiber Crops， Chinese Academy of Agricultural Sciences， Changsha 410205，China） Abstract： In order to find effective methods of extracting extracellular enzymes from Pleurotus eryngii mycelium and provide reliable experimental basis for studying activity of extracellular enzyme and regulating growth of Pleurotus eryngii or other edible fungi， two approaches including acid（citrate buffer at pH 4.8） and distilled water process were used to extract extracellular enzymes from Pleurotus eryngii mycelium. Activities of eight extracellular enzymes were determined to compare the two methods. The results showed that the two extraction methods had significant differences in activity of extracellular enzyme. The activity of Pectinase and cellulase extracted with acid was twice as that of water. The activities of xylanase， pectinase and lignin related enzymes after acid treatment were higher than that of water. The activities of amylase and protease in acid extraction was lower than that in water extraction. Key words： Pleurotus eryngii Qual; extracellular enzymes; acid extraction; water extraction; comparative analysis 研究食用菌胞外酶的活性，对了解不同食用菌的胞外酶分泌特点、活性大小及动态变化趋势、推测不同食用菌在不同生长发育阶段对培养基中木质纤维素、淀粉等大分子营养成分的降解动态、提高栽培技术有重要的意义[1]。在研究胞外酶活性时，能够有效提取菌丝胞外酶是测定酶活性的前提。因此，选择一种有效的提取菌丝胞外酶的方法对研究胞外酶的活性具有重要意义。目前，食用菌胞外酶的提取方法主要有2种：一是用水处理的方式提取[2-4];二是用酸处理的方式提取[5]。到目前为止，尚未见有人用同一种酶对这两种提取方式进行比较分析。由于仪器和试验操作等方面的误差，使得酸性纤维素酶