NgR1在心肺复苏后大鼠海马的表达变化.docVIP

NgR1在心肺复苏后大鼠海马的表达变化 　　【摘要】目的 研究NgR1在CPR后大鼠脑组织中的表达。方法 采用经皮电刺激致心脏骤停方法，60只SD大鼠分组，按照Uestein模式进行CPR，分别于ROSC（restoration of spontaneous circulation）后1 d、3 d和7 d处死动物，利用免疫荧光及Q-PCR方法分别检测脑组织中NgR1蛋白、NgR1 mRNA的分布和变化。结果 CPR后1 d大鼠海马部位NgR1 表达明显升高，CPR后7 d降至正常。Q-PCR结果同免疫荧光细胞计数结果相似，但各时点NgR1 mRNA表达差异并无统计学意义（P0.05）。结论 CPR后大鼠脑组织海马NgR1表达呈现一过性升高，为进一步探讨拮抗NgR1对大鼠CPR后脑功能障碍的作用，寻找改善脑复苏预后的新途径奠定基础。 　　【关键词】NgR1；心肺复苏；海马；大鼠 　　Expression of NgR1 in the hippocampus of rat after cardiopulmonary resuscitation Li Hui， Jing Xiao-li， Li Xin， Hu Chunlin， Wei Hongyan， Xiong Yan， Zhan Hong， Liao Xiaoxing. Department of Emergency， The First Affiliated Hospital of Sun Yat-Sen University， Guangzhou 510080， China 　　Corresponding author：Jing Xiaoli， Email： jing-xl@163.com 　　【Abstract】Objective To investigate the NgR1 expression in the cerebrum after cardiopulmonary resuscitation （CPR） in rats. Methods Cardiac arrest was induced by alternating current in 60 S-D rats through transcutaneous electrical epicardium stimulation， and CPR was performed with Utstein mode at 6 min after CA. Rats were sacrificed at 1， 3 and 7 days after CPR. We performed immunofluorescence with antibodies against NgR1 to map the distribution of NgR1 protein in the hippocampus， while Q-PCR was performed for quantitative analysis of NgR1 mRNA. Results NgR1 expression appeared elevated at 1d and 3 d after CPR and down to the normal level at 7 d in hippocampus. The expression of NgR1 mRNA in hippocampus and cortical cortex showed the same tendency with the staining outcome. The changes were not significant between each time point compared with. baseline in either hippocampus. But there were significant differences between hippocampus and cortical cortex at each time point after the CPR （P0.05）. Conclusions Neuronal NgR1 expression is transient changed during 7 days post-CPR in hippocampus in the rats. 　　【Key words】NgR1； Cardiopulmonary resuscitation； Hippocampus； Rat 　　哺乳动物中枢神经系统（CNS）损伤后神经轴突生长受限可能与一系列神经生长抑制因子有关，如髓鞘相关蛋白（myelin associat