早期分裂三细胞对囊胚形成预测的研究.docVIP

早期分裂三细胞对囊胚形成预测的研究 　　[摘要] 目的 通过观察体外成熟卵母细胞显微注射受精后胚胎特定时间段是否经历早期分裂的三细胞阶段来对其囊胚形成潜能进行预测。 方法 未成熟卵子体外成熟后行卵胞浆内单精子注射（ICSI），授精后33.9～42.9 h内每小时观察1次，记录三细胞胚胎形成数，授精后6 d统计囊胚形成率。 结果 经过三细胞阶段的胚胎囊胚形成率（76.67%）明显高于未出现三细胞阶段（4.17%），差异有统计学意义（P 0.05）；并且在39.9 h之前出现的三细胞胚胎，其囊胚形成率最高。 结论 通过观察胚胎分裂早期是否经历三细胞阶段可对囊胚形成率有较好的预测，为建立有效的、无创的早期预测胚胎发育潜能的指标奠定理论基础。 　　[关键词] 三细胞；早期分裂；囊胚形成；预测 　　[中图分类号] R714.8 [文献标识码] B [文章编号] 1673-7210（2014）10（a）-0069-03 　　Prediction research on blastocyst formation for early division in vitro based on three-cell embryo 　　HUANG Cuiyu DONG Yunqiao CHEN Chuangqi SHI Zhe 　　Department of Reproductive Health and Fertility， Guangdong Maternal and Child Health Care Hospital， Guangdong Province， Guangzhou 511400， China 　　[Abstract] Objective To develop a prediction method of blastocyst formation in vitro by observation the three-cell embryo stage in specific time window of fertilization embryo post intracytoplasmic sperm injection （ICSI）. Methods Human immature oocytes were further cultured until maturity before ICSI was performed. Continuous observation of three-cell stage after ICSI of 33.9-42.9 h were preformed per hour， and the blastocysts rate were recorded post ICSI for 6 d. Results The blastocysts rate of fertilization embryo， which underwent the three-cell stage （76.67%） were higher than those without three-cell stage （4.17%）， and difference between the two groups was significant （P 0.05）. Furthermore， the highest blastocysts rate was identified in fertilization embryo which three-cell stage observed 39.9 h post ICSI. Conclusion The three-cell embryo stage can be used as a potential reference to estimate blastocysts rate of fertilization embryo. 　　[Key words] Three-cell embryo； Early division； Blastocyst formation； Prediction 　　囊胚培养是目前体外培养过程中挑选最具发育潜能胚胎的有效方法[1-2]。但是，囊胚培养体系还不够完善，通过体外受精（IVF）获得的胚胎有50%～70%不能发育为囊胚[3]，因此辅助生殖过程中因无囊胚形成是导致周期取消率升高的主要原因。此外，囊胚培养不可避免地延长了胚胎在体外的生长时间，无疑会导致胚胎基因组及表观遗传的变化。这就需要寻求一种无创且早期预测胚胎发育潜能的指标。在胚胎分裂早期通过观察分裂特性，对体外培养的胚胎的发育做出预测，从而既可早期选择具有发育至囊胚阶段的胚胎，又避免