金属-有机骨架在在线固相萃取技术中的应用和评价.docxVIP

金属-有机骨架在在线固相萃取技术中的应用和评价中文摘要金属-有机骨架（MOFs）是一类新型的多功能型多孔材料，基于MOFs一系列独特的性质，如比表面积大、纳米级孔道、孔道可调控性、化学稳定性和热稳定性良好等，把它应用于样品前处理技术中有很大的优势和潜能。本论文主要以ZIF-8及MIL-101(Cr)为例，探讨MOFs应用于固相萃取技术中。具体内容如下：（1）首先提出将ZIF-8应用于与高效液相色谱联用的在线固相萃取技术中，将其作为吸附剂，选择四环素类抗生素（四环素，土霉素和金霉素）作为分析物，从上样时间、上样流速、pH、盐度和解析时间等方面对分析方法进行探索，并且通过实际水样和牛奶样品考查分析方法的可行性和适用性。结果表明以ZIF-8作为吸附剂的在线固相萃取方法，实现了高效、高选择性地富集四环素类抗生素。此方法具有较宽的线性范围（5-1000 μg L-1）和低的检出限（1.5-8.0 μg L-1，），应用到实际样品中还具有较高的回收率（84.1% 到107.4%）。因此，ZIF-8是一种非常有应用前景的固相萃取吸附剂。（2）其次将MIL-101（Cr）掺杂到有机聚合物整体柱中，将其作为与高效液相色谱联用的在线固相萃取吸附剂，选择间硝基苯酚、间甲基苯酚、邻硝基苯酚和2,4-二氯苯酚作为分析物考查此分析方法。通过探索上样时间、上样流速、盐度和解析时间等参数，优化分析方法。并且以水样为实际样品考查分析方法适用性。结果表明，此分析方法具有反压低、线性范围广、检测限低和回收率高等特点。因此，MIL-101(Cr)掺杂的有机聚合物整体柱是一种有极大的应用前景的固相萃取吸附剂。关键词：金属-有机骨架, 固相萃取, 高效液相色谱， 四环素, 芳香烃AbstractThe unique properties, such as high surface area, uniform structured cavities, permanent nanoscale porosity, have made Metal-Organic Frameworks（MOFs）attractive as the new adsorbent in sample preparation. This dissertation presents the utilization of MOFs as adsorbent for on-line solid phase extraction coupled with High-Performance Liquid Chromatography (HPLC). ZIF-8 and MIL-101(Cr) are taken as the examples. The apllication of MOFs in the solid phase has been explored and summarized as follows:(1) The first example of ZIF-8 as adsorbent for the on-line solid phase extraction coupled with High-Performance Liquid Chromatography was presented. Tetracycline, Oxytetracycline and Chlorotetracycline are chosen as the analytes. We explore some parameters, such as loading time, loading flow rate, pH, salinity and desorption time to optimize the analytical method. The feasibility and suitability of the presented method are also explored through the real samples, water and milk. The result shows that ZIF-8 as the adsorbent for on-line solid phase extraction can extract tetracyclines with high efficiency and excellent selectivity. Under optimized conditions, the developed method gave the linearity range of 5-1000 μg L-1, the detection limits (S/N = 3) of