Enterobacteriaceae GNRs肠杆菌科细菌GNRs.pptVIP

Gram-negative Rods:Glucose nonfermenting  Karen Honeycutt, M.Ed., MT(ASCP)SM MT 418 Clinical Microbiology Student Laboratory Session General Information Predominantly opportunistic Debilitated host Nomenclature changes rapidly (usually known by 2 names for 5-10 years) Will grow on BAP, 22-35°C, 24-72 hours Categories based upon: glucose, oxidase ability to grow on MAC General Information Gram stain morphology taken from non-inhibitory agar Can be difficult to treat, can rapidly develop antibiotic resistance to drugs used for treatment Many genera Commercial system reliability? Glucose “O”, oxidase +, MAC + Pseudomonas sp. Pseudomonas aeruginosa most commonly isolated nonfermenting GNR Grows in moist environments (soap) Aerobic Motile w/polar flagella Pseudomonas aeruginosa Nosocomial infections Usually not part of normal flora Colonization can occur (upper respiratory tract, skin, GI tract) P. aeruginosa - Pigments Usually produces Pyoverdin (fluorescein) – yellow/green; visualize with UV light; Other Pseudomonas sp. can also produce Pyocyanin – blue-green pigment; only produced by P. aeruginosa May produce Pyorubrin – pink to red/maroon pigment; only produced by P. aeruginosa Pseudomonas aeruginosa Id Pseudomonas aeruginosa Id Pseudomonas aeruginosa Rapid Id Pseudomonas aeruginosa infections Cystic fibrosis patients – serious lung infections Immunosuppressed Burn wounds Osteomyelitis UTI, septicemia, Corneal ulcers (blindness) Swimmer’s ear, folliculitis Pseudomonas aeruginosa infections Corneal ulcers (blindness) Pseudomonas aeruginosa infections Burn wound Burkholderia cepacia Nosocomial infections CF patient – colonized/infected with: severe social ramifications; person to person spread established Some CF – once infected with: fulminant decline of pulmonary function Resistant to many antibiotics Glucose “O” or “I”, oxidase (-), MAC + Stenotrophomonas maltophilia Strong maltose oxidizer Nosocomial Opportunistic 3rd most common glucose non-fer