Origin of signals in tissue imaging and spectroscopy组织成像和光谱学中的信号来源.pptVIP

Origin of signals in tissue imaging and spectroscopy组织成像和光谱学中的信号来源.ppt

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Origin of signals in tissue imaging and spectroscopy组织成像和光谱学中的信号来源

Origin of signals in tissue imaging and spectroscopy Andrew J. Berger The Institute of Optics University of Rochester Rochester, NY 14627 A very brief outline Absorption Emission Scattering Who are you? Why are you here? experienced in some branch of optics biomedical not your main shtick interested in survey of fundamentals want introduction to applications interested in following the later talks want pointers to the literature Fred the photon Electronic transitions Vibrational transitions Rotational transitions How to talk about absorption Whats absorbing? Hemoglobin Typical tissue absorption! Hemoglobin Hemodynamics calculations Further adventures of Fred the photon Fluorescence: level diagram A fluorescence scenario The time dimension More introductions to fluorescence Yet more adventures for Fred Level diagram for Raman Basic mechanism of Raman scattering Typical spectrum (oral bacteria) Applications for Raman Fred keeps going, and going, and... Elastic scattering Wavelength dependence varies w/ scatterer size A summary of scattering scales Spectral dependence of scattering Spectral dependence of scattering Scattering spectroscopy Scattering spectroscopy Angularly-resolved scattering Bulk tissue interrogation Absolutely basic photon migration The real deal: diffusion theory What are the diffusion measurements? Still hungry? sphere van de Hulst approximation to Mie theory incident plane wave etalon (F = cavity finesse) d/2 d d=5 microns n1 = 1.36 n2/n1 = 1.06 1-D etalon 3-D sphere wavelength / nm spacing of peaks: size of scatterer depth of modulation: number of such scatterers more rapid oscillations mixture superposition of spectra Perelman et al., Phys Rev Lett 80:627 (1998) and following. normal colon cells cancerous cells broadband polarized illumination polarization-resolved detection d angular distribution has interferometric (oscillatory) behavior as well go to: FTuR1, “Real-time angle-resolved low-coherence interferometry for detecting pre-canc

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