Yeast flotation viewed as the result of the interplay of supernatant composition and cell-wall hydrophobicity.pdfVIP

下载本文档

3
0
约4.03万字
约 11页
2017-03-16 发布于天津
举报
版权申诉

Yeast flotation viewed as the result of the interplay of supernatant composition and cell-wall hydrophobicity.pdf

1、本文档共11页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Yeast flotation viewed as the result of the interplay of supernatant composition and cell-wall hydrophobicity.pdf

Colloids and Surfaces B: Biointerfaces 29 (2003) 309 á/319 /locate/colsurfb Yeast ?otation viewed as the result of the interplay of supernatant composition and cell-wall hydrophobicity Sandro R. deSousa a, Karen F. Oliveira a, Crisla S. Souza a, Beatriz V. Kilikian a, Cecilia Laluce b,* a PPIB-Programa de Po′s-graduac?a?o Interunidades em Biotecnologia-USP/IPT/I. BUTANTAN, Instituto de Cie?ncias Biome′dicas IVUSP, Av. Lineu Prestes 1730, 05508-900 Sa?o Paulo, SP, Brazil b Department of Biochemistry and Technological Chemistry, Instituto de Qu′?mica da UNESP, Universidade Estadual Paulista-UNESP, R. Francisco Degni, s/n, P.O. Box 355, CP 355, 14801-970 Araraquara, SP, Brazil Accepted 19 December 2002 Abstract Flotation is a process of cell separation based on the affinity of cells to air bubbles. In the present work, flotability and hydrophobicity were determined using cells from different yeasts (Hansenulla polymorpha , Saccharomyces cerevisiae , Candida albicans ), which were propagated in different media and at different temperatures. Alterations to the supernatant of the cells were also carried out before the flotation assays. The results described here indicate that supernatants of the yeast cells can play a more important role on flotation than cell-wall hydrophobicity. For example, wall-hydrophobicity of strain FLT-01 of S. cerevisiae was high but flotation did not occur when their washed cells were resuspended in water. Additions of neopeptone to cultures of S. cerevisiae and H. polymorpha repressed flotation and increased the volume of foam. An additional task of the present work was to show that the relationship between cellwall hydrophobicity and flotation performance was dependent on the method used for the measurement of hydrophobicity. Based on the assay procedure, two types of hydrophobicity were distinguished: (a) the apparent hydrophobicity for cells suspended in the medium and expressed by the degree of cell affinity to the organic solvent in the tw