北京汉族群体9个STR位点的频率分布及法医学应用.pdfVIP

-Lf 0— 【 中国法医学袭志1998年第13卷第4期 北京汉族群体9个STR位点的 频率分布及法医学应用 1I乏 y，f 刘雅诚 霍振 唐 晖 杨 剑 北京市岱安局法医检验鉴定中心‘ o∞ j； ； _山 焦章平 提 要 提供北京汉族群体9个STR基因座的频率分布资料．了解其在法医学中的应用价值。 应用PCR技术对9个STR基因座分3组进行复合扩增，经PAG电泳分离、银染。扫描仪扫描，计算 机判读并保存结果，对北京地区汉族无关个体9个基因座的基因频率分布进行调查。结果显示，上述 9个基因座的袭合度为0．6419～0．8092，多态性信息总董为0．9999，鉴别机率为0．9999。匹配机率 为2．0×1O 和非父排除率为0 9985。STR 3组9十基因座的综合检验可应用于法医学个体识别和亲 子鉴定，并达到同一认定的标准。 关键词短申联重复序列(STR) 聚合酶链反应( ! 复合扩增 竺 童 Stady of~lefic frequency distribution of 9 STR loci and its叩pl|eaflo~in f0re呻ic m~llcine practi~e in the Hart population In BeUing／Liu Yacheng，et a1．／Tk Forensic Medical Examination and Id - tiffcation Center ofBe ing，Public Security Bureau，100085 Abstract A PCR multiplexing method of nine STR loci was o_stablished．Nine loci were divided into three groups which were group I (CSFIPO．TPOX，TH01)，group 1I (F13A01．FESFPS，vWA)and groupⅢ (D16S539，D7S820，D13S317)．The muhi- plexing method was camed out consequently as PCR，PAGE．silver staining，scanning． reading and saving the resuIts in the computer．The allele frequency distribution of nine STR loci of 195～236 unrelated individuals of Han pop~ations in Beijing has been studied．The hetmaygosity(H)，discriminating power(Dp)．probability of exclusion(PE)，polymor— phism information content(PIC)and the probability of matching were 0．6419～0．8092， 0．9999．0．9985．0．9999 and 2．0 x 10 9 respectively． It is suggested that this multiplex— ing of nine STR loci can be used in personal identification and paternity testing． Key words Short tandem repeat(STR) Polymerase chain reaction(PCR) Mul- tiplexing Paternity testing Personal identification STR的遗传多态性被广泛应用于法医学 在常规的办案中应