an immunochromatographic strip test for therapid detection of okadaic acid in shellfish sample.pdfVIP

下载本文档

13
0
约3.51万字
约 9页
2017-04-06 发布于江苏
举报
版权申诉

an immunochromatographic strip test for therapid detection of okadaic acid in shellfish sample.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

an immunochromatographic strip test for therapid detection of okadaic acid in shellfish sample

Development of an immunochromatographic strip test for the rapid detection of okadaic acid in shellfish sample Leqin Hu Junxiu Liu Qing Wang Yang Zhang Rui Jia Chuner Cai Weining Wu Steven-Feng Chen Peimin He Received: 15 April 2012 /Revised and accepted: 15 November 2012 /Published online: 24 February 2013 # Springer Science+Business Media Dordrecht 2012 Abstract A rapid detection technology for okadaic acid (OA) in shellfish with one-step immunochromatographic assay using colloidal gold-labeled monoclonal antibody (Mab) probe was developed. OA is one of the diarrhetic shellfish toxins. Firstly, OAwas conjugated to bovine serum albumin, and the conjugations as immunogen were injected into mice to raise the polyclonal antibody against OA. Hybridoma cells fused between spleen cells from immu- nized mouse and myeloma cells (Sp2/0) were prepared and injected into mice intraperitoneally at 1×106cells to produce monoclonal antibody in the ascitic fluid. With the monoclo- nal antibody against OA, the idc-ELISA assay was estab- lished to detect OA. The calibration curve for OAwas linear over the concentration range of 0.31–50 ng mL?1, and the detection limit for OA was 0.45 ng mL?1. On that basis, paper test strips for detecting OA were prepared, and a fast detection method for okadaic acid using gold-labeled im- munological assay was established. With the paper test strips, the detection limit was 6.25 ng mL?1, and whole detection process for OA in shellfish samples needed only about 40 min. Keywords Diarrhetic shellfish poison (DSP) . Okadaic acid (OA) . Antibody . Enzyme-linked immunosorbent assay (ELISA) . Colloidal gold, paper test strip, fast detection Introduction Okadaic acid (OA) is a lipophilic marine algal toxin, which was first extracted from the sponges Halichondria okadai and Halichondria melanodocia (Tachibana et al. 1981). Later it was found to be the same toxin as that isolated from the dinoflagellates Prorocentrum and Dinophysis (Murakami et al. 1