直立穗型粳稻不同粒位籽粒发育的差异.pdfVIP

沈阳农业大学学报，2014-10 ， 45(5) :518-522 Joumal of Shenyan只 A只ricultural University ,2014-10 ,45(5) :518-522 直立穗型梗稻不同粒位籽粒发育的差异 程海涛 1，呼楠 l气吕文彦 l*，曹萍 l (1.沈阳农业大学农学院，沈阳 110161:2 内蒙古鄂尔多斯市杭锦旗科技局，内蒙古鄂尔多斯 017400) 摘要:以直立大穗型梗稻为试材，经自上而下梯度剪除稻穗枝梗的梯度减库处理，比较稻穗上、中、下部位的 1 次枝梗(强势粒)和 2 次枝梗(弱势粒)子粒胚乳细胞发育差别。并以 Iρgistic 方程模拟不同粒位子粒的胚乳细胞增殖过程，研究细胞数与粒重在发育过 程上的相关性。结果表明:梯度减库后，中下部强势粒总细胞数明显提高，与未减库的对照顶端强势粒水平相近，但下部弱势粒细 胞数目始终不及强势粒，与顶端强势粒相差最大;不同穗粒位子粒细胞增殖起始势(~)呈现上部〈中部〈下部，强势粒〉弱势粒的趋 势，而细胞有效增殖期 (D)表现为上部〉中部〉下部，强势粒〉弱势粒的趋势，减库处理相应粒位的~降低，D 增加;下部强势粒和弱 势粒的粒重差异不随减库强度的增加而显著减小;单粒胚乳细胞数与单粒重极显著正相关，弱势粒单粒重与~和各增殖时期持 续时间显著相关。综上可见，上部强势粒和下部弱势粒充实程度(粒重)存在差异，这种差异可能与弱势粒胚乳细胞增殖速度慢、最 终数目少有关，通过减库增源能使下部弱势粒粒重显著增加，但弱势粒发育仍达不到强势粒水平，说明库源关系对弱势粒发育有 影响，但弱势粒本身库活性才是制约自身发育的主要因素。 关键词:梗稻;直立穗型;粒位;胚乳发育;胚乳细胞 DOI: 1 0.3969/j .issn.l 000-1700.20 14.05.002 中图分类号: S51 1.2.2 文献标识码 :A 文章编号 :1000-1700(2014)05-0518-05 Developmental Differences among Different Positions Grains in Japonica Rice with Erect Panicle Type CHENG Hai-Tao 1 , HU Nan 1 ,2, Lü Wen-Yan 1 *, CAO Ping 1 (1.College of Agronomy, Shenyang Agricultural University, Shenyang 110161 , China; 2. Ordos City of Inner Mongolia Autonomous Region HangJin County Science Technology, Ordos Inner Mongolia 017400, China) Abstract: Researches showed that the insufficient of nutrition supply was one reason for poor development of endosperm in rice, however, how much does this suppression effect has, was not very clear. So, a EPT-rice with large-spike cultivar was selected as material to compare the development of endosperm between the grains of first and second branches using gradient reducing sink treatment. A Logistic equations was used to simulate endosperm cell proliferation of grains on different pa巾。，f rice panicle, for researching the correlation between grain weight and the number of endosperm cell. The results showed: The number of endosperm cells at superior grain of upper parts of a panicle improved with the increasing of source -sink ratio after the treatment of reducing sink. It could