Asia1型FMDV感染BHK-21细胞差异蛋白质点2-DE分析.pdfVIP

畜牧兽医学报 2011.42 (1) :145町149 Acta Veterinariα et Zootechnica Sinica Asia 1 型 FMDV 感染 BHK-21 细胞差异 蛋白质点 2-DE 分析 刘永杰，张Jt山，尚佑军，郭建宏，郑海学，田 宏，卢国栋，刘湘涛将 (中国农业科学院兰州兽医研究所，家裔疫病病原生物学国家熏点实验攘，农业部裔禽病毒学景点汗放实验室， 罔家口蹄疫参考实验室，兰州I 730046) 摘 要:为研究 Asia 1 糊口蹄疫病毒(FMDV)感染 BHK→21 细胞后蛋白质组的变化及差异，将 FMDV 接种于培养 的单层 BHK-21 细胞，同时设米接毒的 BHK-21 细胞作对照级。待细胞病变后分别提取 2 细细胞总蛋白借助 2翩。在 技术及 PDQuest8.0 软件进行细胞蛋白质组工维电泳阁谱差异性分析。结果显示感染组有 1 457 个可见蛋白质 点，而对照组可见的蛋白质点为 1 427 个。!感染组蛋白点的表达量与对照组相比，二者具有统计学差异 (P0.05) 的蛋白质点 472 个，其中表达上调 251 个点，表达下调 221 个点，新合成蛋白点 30 个。结果表明 FMDV 感染 BHK 21 细胞后引起了细胞强白质组表达模式的改变，这种变化是病毒与细胞相互作用的结果。本研究首次利用蛋白质 组学技术研究 Asia 1 型 FMDV 感染 BHK-Z1 细胞后蛋白质组学差异，有助于深入研究 Asia 1 型 FMDV 和 BHK→ 21 细胞村|句:作用的分子机制。 关键词: Asia 1 型 FMDV; BHK-21 细胞;感染;差异蛋白质组 中图分类号: S852. 659. 6 文献标识码 :A 文章整编号: 0366-6964(2011)01-0145-05 Two-dimentional Gel Electrophoresis Analysis of Differential Protein Spots from BHK-21 Cell Infected FMDV Serotype Asia 1 LlU Yong-jie , ZHANG Ke-shan , SHANG You-jun , GUO Jian-hong , ZHENG Hai-xue , TIAN Hong , LU Guo-dong , LIU Xiang-ta旷 (State Key Laboratory of Veterinary Etiological Biology , Key Laboratory of Animal Virology 。fMinistry of Agriculture , National Foot巾an叶Mouth Disease Reference Laboratory , Lanzhou Veterinary Rese，αrch Institute , Chinese Academy of Agricultural Sciences , Lanzhou 730046 , China) Absírací: 丁o examine the differences and changes of proteome of BHK-21 cells after infection with foot-and-mouth disease virus CFMDV) serotype Asia 1 , the monolayer BHK-21 cells infec- ted with FMDV and normal cells were prep昂red as experimental materials. The total protein from the BHK-21 cells were isolated and used to analyze the proteome differences between the infected and the control cells by 2-dimentional gel electrophoresis (2四日E). Results showed that there were 1 457 protein spots visible in the infected cells , and 1 427 visible protein spots in the control cells. Compared with controI cell日， there were significance of statistics CPO. 05) at 472 differential protein spot