南开大学《遗传学》SOLiD_产品介绍.pptVIP

SOLiD? System OverviewABI 新一代高通量基因分析系统;Applied Biosystems SOLiD? System – 概况;SOLiD – 工作流程;Workflow;DNA Fragment 60-90 Bases;构建随机片段文库 ;构建配对末端文库 (i)?;Mate-paired libraries (ii)?;Mate-paired Libraries (iii)?;油包水PCR: Emulsion PCR (ii)?;Emulsion PCR (ii)?;Emulsion Metrics Bead size: 1 ?m Reactor size: 4 ?m Reactor volume: 34 fL Beads / emulsion plate (96-well): 2-4 x 109 Post Enrichment: ~500M / plate;emulsion PCR Individual Bead;emulsion PCR Individual Bead;Beads collected following emulsion PCR:;纯化 Enrichment;P2-hybridization, Pre- and post-enrichment;测序芯片 Sequencing Array;  Pro