碳源对卷枝毛霉EIM-10 γ-亚麻酸产量的影响 (1).pdfVIP

74 生 物 技 术 2011年 2l(2) 碳源对卷枝毛霉 EIM—lO 一亚麻酸产量的影响 严建平，江贤章 (工业微生物教育部工程研究中心，福建师范大学生命科学学院，福建省现代发酵技术工程研究中心，福建 福州350108) 摘要：目的：研究碳源对卷枝毛霉脂肪酸产量的影响，为代谢调控卷枝毛霉生产 一亚麻酸奠定基础。方法：测定卷枝毛霉 在各种碳源、碳源浓度及碳氮比条件下生物量、油质产量及油脂中GLA含量。结果：卷枝毛霉EIM一10在以葡萄糖为碳源发酵 时，油脂得率为2％，油脂中 一亚麻酸含量为18％；以大豆油为碳源时，其生物量(干重)达到33 L，油脂占菌丝体干重的35％， GLA的含量为3％。卷枝毛霉EIM一10不能利用醋酸和柠檬酸，可以利用醋酸钠和柠檬酸钠生长但不积累油脂。结论：卷枝毛 霉ElM一10脂肪酸从头合成能力不强，能利用外界脂肪酸合成细胞内油脂。 关键词：卷枝毛霉 ； 一亚麻酸；多不饱和脂肪酸；碳源 中图分类号：Q935 文献标识码：A doi：10．3969／j．isn．1004—311X．2011．02．054 Influence of Carbon Sources on —linolenic Acid Production by Mucor circinelloides E1M 一1O YAN Jian—ping，JIANG Xian—zhang (Engineering Research Center of Industrial Microbiology，Ministry of Education，Colege of Life Science，Fujian Nomlal University， Engineering Research Center of Fujian Modem Fermentation Technology，Fuzhou 350108，China) Abstract：Objective：To study the influence of carbon sources one／一linolenic acid production by Mucor circineloides EIM一10 and make a solid foundation for regulating GLA producing of Mucor circinelloides EIM 一10．Method：Dry cell weight and lipid yield of mucor circinel— loides and GLA of the oil content was determinated．Result：While glucose was used as carbon source．only 2％ lipid of its dry cel weight which contained 1 8％ 一linolenic acid was accumulated by Mucor circineUoides EIM 一10；While soybean oil was used as carbon source， the biomass of Mucor circineUoides was 33s／L and the lipid content was 35％ of its dry cell weight with a GLA content of 3％．Acetic acid and citric acid could not be utilized but sodium acetate and sodium citrate could be utilized without lipid accumulation by Mucor circinel— loides EIM 一10．Conclusion：Ectogenesis fatty acid could be utlized to accumulate lipid but little fatty acid could be synthesized to produce oi1 by the strain Mucor circineloides ElM —lO． Key words：Mucor circineloides； 一linolenic acid；polyunsaturate faty acid；carbon source 0 引言 一 亚麻酸( 一linolenic acid，GLA)为全顺式6，9，12一十 八碳三烯酸，属于 ∞一6系列多不饱和脂肪酸，是人体的一种 必需脂肪酸。GLA具有抗菌、抗癌、抗动脉粥样硬化等作