临床医学英语论文.doc

Abstract: objective to study of TNF alpha Ghrelin human brain microvascular endothelial cells induced (HBMEC) mononuclear cells chemotaxis protein 1 (MCP-1) influence and p38MAPK role. Methods HBMEC training, TNF alpha group to join different concentration TNF alpha; Ghrelin pretreatment section first join Ghrelin pretreatment 1 h, then add the TNF alpha. The RT-PCR method to detect MCP-1 mRNA level, immune imprinting method to detect p38 phosphorylation (p-p38) expression. The results of the TNF alpha stimulation, MCP-1 mRNA express obvious enhancement, and the concentration of a certain dose-response relationship (P 0. 05) in the cytoplasm and P-p38 protein expression also significantly increased (P 0. 05). Ghrelin pretreatment can reduce MCP-1 mRNA cytoplasm and the p-p38 protein expression (p 0. 05). Conclusion Ghrelin may inhibit p-p38 pathways inhibit TNF alpha mediated HBMEC MCP-1 mRNA expressions. Keywords: mononuclear cells chemotaxis protein 1; The human brain microvascular endothelial cells; Ghrelin In recent years, and the relationship between Ghrelin inflammation is paid attention to, but for inflammation, Ghrelin in different tissues function is not the same, need to make clear further [1 ~ 5]. April 2008 to July 2009, we studied Ghrelin TNF alpha induction of the human brain microvascular endothelial cells (HBMEC) mononuclear cells chemotaxis protein 1 (MCP-1) phosphorylation mRNA and p38 (p-p38) the influence of the expression, this paper discusses Ghre-Lin in the possible anti-inflammatory mechanism vessel, as Ghrelin applied to clinical diabetes cerebrovascular disease and provided the reference. 1 materials and methods The human brain microvascular endothelial cell lines 1 materials HBMEC Scien cell bought in the United States, rhTNF alpha Sigma company bought in, TaKaRaRNA PCR Kit (AMV) Ver. 3. 0 bought in dalian treasure biological engineering Co., LTD, p-p38MAPK mice monoclonal antibodies against human bought in Santa Cruz company, other dr