利用RAMP分子标记对酸枣资源的遗传多样性分析.PDF

第 32 卷 第 2 期 经 济 林 研 究 Vol. 32 No.2 2014 年 6 月 Nonwood Forest Research Jun. 2014 利用 RAMP 分子标记对酸枣资源的遗传多样性分析 张建英 1,2，毛向红 1,2，张莹莹 1,2 （1. 河北省林木良种工程技术研究中心，河北 石家庄 050061；2. 河北省林业科学研究院，河北 石家庄 050061） 摘 要：为了探讨不同区域酸枣资源的遗传多样性，完善酸枣的 DNA 分子指纹鉴定方法，用 24 对引物对 31 份 酸枣样品进行 RAMP 分子标记分析，从中筛选出 14 条有多态性并且条带清晰的引物，共扩增了 132 个位点，多 态性位点 107 个，多态性比率 81.1%。遗传多样性分析结果表明，31 份酸枣样品的遗传距离为 0.120 9 ～ 0.517 2； 根据扩增结果可将参试的 31 份样品划分为 6 类。 关键词：酸枣；遗传多样性；亲缘关系；RAMP 标记；聚类分析 中图分类号：S665.1 文献标志码：A 文章编号：1003—8981(2014)02—0014—05 Analysis on genetic diversity in Ziziphus acidojujuba resources based on RAMP markers ZHANG Jian-ying1,2, MAO Xiang-hong1,2, ZHANG Ying-ying1,2 (1. Hebei Engineering and Technology Research Center of Improved Forest Variety, Shijiazhuang 050061, Hebei, China; 2. Hebei Academy of Forestry Science, Shijiazhuang 050061, Hebei, China) Abstract: In order to research genetic diversity in Ziziphus acidojujuba resources at different regions and improve the technology of DNA molecular fingerprint identification in Z. acidojujuba, 31 samples in Z. acidojujuba were analyzed by using a total of 24 primers and RAMP molecular markers techniques. 14 primers with polymorphic and clear bands were screened out. 132 loci were amplified, among which 107 loci were polymorphic, and the polymorphic percentage was 81.1%. The results of genetic diversity analysis