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Alligator grass-butanol part of high-performance liquid chromatography fingerprint
Author: Wang Jia Xin, and Chen Shu-Fang Nian-Bo, Liu Yan-Wen
[Abstract] Objective To establish a grass-butanol Alligator parts of the high-performance liquid chromatography (HPLC-ELSD) fingerprints. Methods HPLC-ELSD determination, the mobile phase of acetonitrile - water gradient elution, flow rate of 1.0 ml * min-1, evaporative light scattering (ELSD) detector, column temperature was 35 ℃ . The results of the use of gradient elution can be better to separate parts of Alligator grass-butanol compounds; by the methodological study, HPLC-ELSD fingerprint with good repeatability, initially established a total of 12 peaks characterized by HPLC-fingerprint information ELSD fingerprints. Conclusion The Alligator grass-butanol parts of the HPLC-ELSD fingerprint analysis for quality evaluation of Alligator grass provide a reference.
[Keywords:] Alligator grass; fingerprinting; high-performance liquid chromatography; evaporative light scattering detector
Abstract: ObjectiveTo establish the HPLC-ELSD of n-butylol froution of Altemanthera philoxeroide.MethodsThe gradient elution was adopted with a mixture consisting of acetonitrile and water, the flow rate was 1.0 ml * min-1, and the detector was ELSD. ResultsThe constituents of Altemanthera philoxeroide were well separated on HPLC and the HPLC-FPC of 10 batches of samples was established.The results showed that this method had a good repeatability and the 12 common peaks had an exclusive properties.ConclusionThe method developed can supply the reference for the quality evaluation of Altemanthera philoxeroide.
Keywords:: Altemanthera philoxeroide; Fingerprint chromatography; HPLC; ELSD
Alligator grass Altemanthera philoxeroides (Mart.) Griseb for the Amaranthaceae (Amaranthaeeae) Alternanthera Ahemanthera perennial perennial herb, also known as hollow Amaranthus, crab trilobata, water, peanuts, Alternanthera
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