As2O3 induced apoptosis in human ovarian cancer cell lines HO8910 and telomerase activity in.docVIP

 PAGE \* MERGEFORMAT 14 As2O3 induced apoptosis in human ovarian cancer cell lines HO8910 and telomerase activity in Of: PROCEEDINGS high wind, Hao Zhiming, Wang Ying [Abstract] Objective HO8910 As2O3 induced apoptosis and changes in telomerase activity. Methods with different concentrations of As2O3 solution with ovarian cancer cell line HO8910, cells were collected at different time points, MTT cell growth inhibition assay, FCM Identification of Apoptosis, TRAP-silver staining of telomerase activity were observed. Results As2O3 solution on HO8910 growth inhibition and apoptosis induction has the role of drug concentration and time dependent. different concentrations of As2O3, HO8910 cells, telomerase activity in 24 h no significant change, with the effect of time, telomerase activity decreased, with the increase of telomerase activity in drug concentration was also declining trend, which disappeared. Conclusions As2O3 can induce ovarian cancer cell line HO8910 apoptosis, which may not directly regulated by telomerase activity, but the regulation of telomerase regulation and apoptosis have some relevance. [Keywords:] as arsenic trioxide, human ovarian cancer cells, apoptosis, telomerase activity ABSTRACT: Objective To explore the relationship between the variation of telomerase activity and apoptosis by arsenic trioxide in ovarian carcinoma cell line HO8910. Methods We collected HO8910 cells at 24 h, 48 h and 72 h respectively under different concentrations of As2O3. The inhibition rates of cells were determined by methyl thiazolyl tetrazolium (MTT) assay to evaluate the effect of As2O3. The percentage of apoptosis was detected by flow cytometry (FCM). Telomerase activity of ovarian carcinoma cells was determined by the method of TRAPargentation. Results Growth inhibition and apoptosis induction were evoked by different concentrations of As2O3 in HO8910 cells in a time-and concentration-dependent manner. Within 24 h, the telomerase activity of HO89