Corn husk on vascular endothelial smooth muscle apoptosis and expression of the medicinal effects of p53.docVIP

 PAGE \* MERGEFORMAT 9 Corn husk on vascular endothelial smooth muscle apoptosis and expression of the medicinal effects of p53 OF: DING Meng, Li pull source with Wang Hui, Li Yi, Wang Zhijun, Zhen Yanjun [Abstract] Objective To study the corn husk on high blood vascular endothelial and smooth muscle cells with mutant p53 expression in the medicinal effects. Methods Male SD rats were randomly divided into normal control, pathological model, positive control and corn bract decoction group, copy the prey hyperlipidemia, preventive administration of decoction of corn husk to Gemfibrozil for the positive control. flow cytometry endothelial and smooth muscle cell apoptosis and p53 mutation expression. Results of corn husk decoction group of mutant p53 expression in endothelial cells decreased (Pgt; 0.05), the apoptosis rate decreased (P lt;0.05), proliferation index increased slightly (Pgt; 0.05); smooth muscle decreased expression of mutant p53 (P lt;0.05), the apoptosis rate increased (P lt;0.05), proliferation index decreased (P lt;0.05). Conclusion decoction of corn husk with a control rat vascular endothelial and smooth muscle cell p53 expression, apoptosis and balance the two correlation between the proliferation of medicinal effects. [Keywords:] corn husk; endothelium; smooth muscle; mutant p53 Decoction of corn husk with anti-atherosclerosis (Atherosclerosis, AS) of the medicinal effects of many experimental results have been confirmed by us [1 ~ 3]. In this paper, high blood fat rat aortic endothelial cells (endothelial cells, EC) and smooth muscle cells (smooth muscle cell, SMC) proliferation index and apoptosis rate and expression of mutant p53 correlated corn husk to reveal the role of early injury intervention in AS. 1 Materials and equipment 1.1 cleansing of drug collected mature corn husk, dried, chopped corn husks per gram of boiled distilled water 100 ml 3 h, concentrated to 1.5 g / ml crude drug use. Use of sodium carboxymethyl ce