Decoction of Bupleurum and saponins of HPLC fingerprint.docVIP

 PAGE \* MERGEFORMAT 9 Decoction of Bupleurum and saponins of HPLC fingerprint [Abstract] Objective HPLC fingerprint technology, comprehensive before and after boiling Bupleurum reveal changes in saponins. Methods Hypersil C18 column (250 mm 4.6 mm, 5 m); mobile phase of acetonitrile - water, gradient elution [0 ~ 70 min: acetonitrile - water (22:78) (50:50)]; flow rate of 1.0 ml min-1; column temperature 30 ; detection wavelength of 210,252 nm. Results after Bupleurum decoction in the fingerprint there does not exist in 9 of the new medicine peaks. Conclusions Bupleurum decoction in the emerging system of saponins in-depth study, from the Chinese point of view will help to reveal the material basis for the efficacy of Bupleurum. [Keywords:] Bupleurum; decoction; saikosaponin; high performance liquid chromatography; fingerprint Abstract: ObjectiveTo disclose the changes of saikosaponins between Radix Bupleuri and its water extract by HPLC fingerprint technology. MethodsThe HPLC fingerprint was performed on a Hypersil C18 column (250 mm 4.6 mm, 5 m) with gradient elution of Acetonitrile - Water [0 - 70 min: Acetonitrile - Water (22:78) (50:50)], the flow rate was 1.0 ml min-1, the column temperature was set at 30 , and the detection wavelength was 210 and 252 nm. ResultsNine peaks which did not exist in Radix Bupleuri were found in its water extract. ConclusionFurther research should be carried on the nine new components appeared in the water extract of Radix Bupleuri to explain the functions of Saiko. Keywords:: Radix Bupleuri; Water extraction; Saikosaponin; HPLC; Fingerprint Has been reported that the boiling process Bupleurum saponin in the existence of structural changes in composition [1], but people in the extraction process Bupleurum study, tend to adopt various measures to prevent the occurrence of such changes, such as alkaline solvent extraction [2], low concentration [3], percolation [3], the above measures, although improved sai