DNAPKcs expression and radiosensitivity of nasopharyngeal carcinoma cell lines the relationship between the.docVIP

 PAGE \* MERGEFORMAT 6 DNAPKcs expression and radiosensitivity of nasopharyngeal carcinoma cell lines the relationship between the Author: Qu Song, ZHU Xiao-dong, Dan-Rong, Zhang Wei, Cao Ji [Abstract] Objective To investigate the radiosensitivity of nasopharyngeal carcinoma cell line CNE  1 (well-differentiated nasopharyngeal carcinoma cell line) and the CNE  2 (nasopharyngeal carcinoma cell line), the DNA-dependent protein kinase (DNA  PK ) catalytic subunit of DNA  PKcs gene expression and the relationship between the radiosensitivity of nasopharyngeal carcinoma cells. Methods Experimental Determination of colony forming CNE  1, CNE  2 different doses of survival fraction, and use the linear quadratic model fitting dose-survival curve derived radiobiological parameters α , β , SF2, MID values, as well as the blue tetrazolium colorimetric assay (MTT) test 60Co  γ line 4Gy cell survival 12h after irradiation to evaluate the two cell radiosensitivity. Real-time fluorescence quantitative reverse transcription-PCR (RT  FQ  PCR) detection of irradiation before and after different time and different doses of CNE  1, CNE  2 cell mRNA levels of DNA  PKcs quantitative gene expression. Results CNE  1 in each dose point survival fraction than that CNE  2 high, MID values were 2.78,1.61, SF2 values were 0.627,0.341; 4Gy survival rate 12h after irradiation, respectively, 88.2%, 72.3%; RT  FQ  PCR showed two cells have the DNA  PKcs gene expression, their relative expression level of the ratio of 7.54 ± 2.71 (t = 4.17, P = 0.014), expression of the difference was statistically significant, DNA  PKcs gene in the CNE  2 cells, there is time and dose-dependent manner. Conclusion experimental verification of the CNE  2 compared with CNE  1 is more sensitive to rays, DNA  PKcs gene expression and radiosensitivity of nasopharyngeal carcinoma cells relevant. [Keywords:] nasopharyngeal carcinoma cells; real-time fluorescence quant