DNMT1 catenin and PGSK3 expression in colon cancer.docVIP

 PAGE \* MERGEFORMAT 13 DNMT1 catenin and PGSK3 expression in colon cancer Of: Richard Winter long, He Dalin, Zhao Jiahui, Baiji Rong [Abstract] Objective To study the DNA methyltransferase 1 (DNMT1), catenin ( catenin) and phosphorylation of glycogen synthase kinase (P GSK 3) in colon cancer and tumor differentiation and lymph node metastasis and so on. Methods detected by fluorescence quantitative PCR in 62 cases of primary colon cancer tissues and 21 cases of normal colorectal mucosa DNMT1, catenin expression and GSK by Westernblot method validation expression. Results colon cancer of DNMT1, catenin and P GSK 3 expression level was significantly higher than normal tissue. DNMT1 levels in poorly differentiated between the relatively high content of cancer tissue, catenin levels in tumor differentiation and lymph node metastasis of the cancer between the relative content significantly, while the group P GSK 3 was no significant difference. Conclusion DNMT1, catenin and P GSK 3’s activity and colon cancer have a certain relevance. [Keywords:] DNA methyltransferase 1, the phosphorylation of glycogen synthase kinase, catenin, colon cancer ABSTRACT: Objective To examine DNMT1, catenin and P GSK 3 expressions in tissues of colon carcinoma compared with tumor clinicopathological parameters including differentiation and metastasis. Methods SYBR Green real time PCR and immunoblotting method were used to detect these gene expressions in 62 tissues of colon carcinoma and 21 tissues of normal colon. Results Overexpressions of DNMT1, catenin, and P GSK 3 were found in colon carcinoma tissues. The expression of DNMT1 was found to be higher in poorly differentiated tissues. The results also demonstrated statistical significance in the expression of catenin involving differentiation and metastasis, but no statistical significance in the expression of P GSK 3. Conclusion These results show that DNMT1, catenin and P GSK 3 expressions are regulated through