Donor lymphocytes in GVHD mice organs and tissue migration and distribution of dynamic observation of.docVIP

 PAGE \* MERGEFORMAT 3 Donor lymphocytes in GVHD mice organs and tissue migration and distribution of dynamic observation of Authors: WEN Sheng, Wang Jian-Min, Zhou Hong, Xia, Qiu Huiying, Lei Gao, Xiao-Xia Hu [Abstract] In this study, mice with GVHD model of allogeneic T lymphocytes in transplant recipients in vivo migration and distribution. The C57BL / 6 bone marrow cells and transgenic fluorescent C57BL / 6 mice spleen lymphocytes importation of 8 Gy total body irradiation of BALB / c mice, the establishment of donor lymphocyte marker eGFP mouse model of GVHD; application of fluorescence microscopy and flow cytometry observation GVHD model the distribution of eGFP cells; ELISA assay GVHD target tissue chemokine MIP-1α levels change. The results showed that: ① Input spleen cells and bone marrow cells in the first eight days after the clinical and pathological manifestations of GVHD occurred; ② GVHD model, recipient rats liver, skin, intestines, spleen, lung, and tongue with eGFP cell infiltration; ③ GVHD in mice liver and spleen eGFP cells, CD4, CD8 cell ratio were gradually increased; ④ GVHD spleen and liver tissues elevated levels of MIP-1α , MIP-1α levels in the spleen peaked at 3 days after transplantation, the liver levels of MIP-1α peaked on the 7th day after transplantation. Conclusion: In addition to the liver, intestines, skin, the lungs, the tongue may also be the target organ GVHD. Liver and spleen tissue donor lymphocyte infiltration accompanied by elevated levels of MIP-1α . [Keywords:] mouse GVHD model; T lymphocytes; enhanced green fluorescent protein; macrophage inflammatory protein Migration and Distribution of Allogeneic T Lymphocytes in Organs of Graft-Versus-Host Disease Mouse Model AbstractThis study was aimed to investigate the migration and distribution processes of allogeneic donor T lymphocytes in the organs of recipient mice. GVHD model was established by transfusion of the splenocytes of eGFP transgeneic C57BL / 6 mic