Doxorubicin on human breast cancer cells MCF7 Inhibition of the expression of KIF4A.docVIP

 PAGE \* MERGEFORMAT 11 Doxorubicin on human breast cancer cells MCF7 Inhibition of the expression of KIF4A Of: Wang Lu, Wu Huijuan, Xu Ning, Wang Cong Yang, Ho Chak, Shen Chuan Lu [Abstract] Objective: To investigate the adriamycin on the expression of human breast cancer cells MCF 7 KIF4A. Methods: Using Western blotting to detect MCF 7 cells with adriamycin treatment of the cells at different time after the KIF4A protein; with different concentrations of A adriamycin treatment MCF 7 cells after 96 h, detection KIF4A protein, and the age-related galactosidase staining. Results: with and without treatment with doxorubicin compared to MCF 7 cells, doxorubicin treatment of MCF 7 cells KIF4A protein decreased; age-related galactosidase staining from 5% to 42% of cells were positive for senescence-like morphological change was also found. Conclusion: MCF 7 cells with adriamycin treatment could reduce the level of protein expression KIF4A, KIF4A protein decrease with doxorubicin induced senescence in MCF 7 cells needs further study. [Keywords:] KIF4A protein; doxorubicin; cell senescence; MCF 7 cells [Abstract] Objective: To study the effects of adriamycin on expression of KIF4A protein in MCF 7 cells. Methods: During different periods of time after the cells treated with adriamycin, the expression level of KIF4A protein was detected by Western blotting. Then, 96 h after the MCF 7 cells were treated with different concentrations of adriamycin, the expression of KIF4A protein was monitored by Western blotting, and at the same time the senescent MCF 7 cells were measured by using the method of senescence associated galactosidase stain. Results: Compared with the untreated MCF 7 cells, the expression level of KIF4A protein in the MCF 7 cells treated with adriamycin was significantly decreased. The MCF 7 cells positive for the senescence associated galactosidase marker were increased from 5% to 42% by 1 mol L-1 of adriamycin. Meantime, the positive stain