Doxycycline in human lung cancer cell line proliferation and invasion behavior.docVIP

 PAGE \* MERGEFORMAT 14 Doxycycline in human lung cancer cell line proliferation and invasion behavior [Abstract] Objective of doxycycline on human lung cancer cell line A549 cells and in vitro invasion and matrix metalloproteinase -2 (MMP-2) expression. Methods MTT assay of different concentrations of doxycycline ( 2.5,5,10,20,50 mg / L) on A549 cell proliferation, immunohistochemistry and Millicell-pore chamber method, different concentrations of doxycycline (2.5,5,10,20 mg / L) process after A549 cells MMP-2 protein expression and in vitro invasive ability. The results 10mg / L doxycycline than A549 cells inhibited, 5mg / L doxycycline than A549 cells inhibited MMP-2 protein expression and in vitro invasion. Conclusion Doxycycline can inhibit proliferation and in vitro invasion of A549 cells, the inhibition of invasion and metastasis of A549 cells The inhibition of MMP-2 expression. [Keywords:] lung cancer invasion and matrix metalloproteinase doxycycline Abstract: Objective To study the effects of doxycycline on the proliferation, expression of matrix metalloproteinase-2 (MMP 2) and the invasiveness of lung cancer carcinoma cell line A549 in vitro. Methods MTT assay was applied to study the effects of doxycycline (2.5,5,10,20,50 mg / L on the prolifera tion of A549. Immunohistochemistry stain and millicell pore chamber were used to investigate the expression of MMP 2 and the invasive ability of different concentration of doxycycline (2.5,5,10,20 mg / L treated A549 cells. Results Doxycycline more than 10mg / L inhibited the proliferation of A549 in a nose and time dependent manners. Doxycycline more than 5mg / L inhibited the expression of MMP 2 and invasivenessn of A549. Conclusion Doxycycline can inhibit the proliferation, invasiveness and metastasis of A549 and the mechanism of which is related to the inhibition of MMP 2 in A549 cel1. Keywords:: lung cancer, matrix metalloproteinase, doxycycline; invasiveness Lung cancer is a very high mo