Effects of estrogen on ovariectomized hyperlipidemic C57BL - 6 mice the expression of ZFP580 mRNA.docVIP

 PAGE \* MERGEFORMAT 10 Effects of estrogen on ovariectomized hyperlipidemic C57BL / 6 mice the expression of ZFP580 mRNA On: Sun Huiyan Weng Lijun Zhao Yanling Chen Shao-yu Zhang Xu Wei Shuping Zhang Wencheng [Abstract] Objective To study the effect of estrogen on C57BL / 6 mouse aorta ZFP580 gene expression. Methods sham, ovariectomized group (OVX, ovariectomized experimental group (ovariectomized and estradiol benzoate intraperitoneally diol C57BL / 6 mouse model of hyperlipidemia and the identification, extraction of C57BL / 6 mouse aorta total RNA, were designed and synthesized ZFP580 and actin gene primers, using semi-quantitative RT PCR analysis of mRNA levels of ZFP580. Results constructed successfully castrated C57BL / 6 mouse model of hyperlipidemia, ovariectomized control mice ZFP580 gene expression level with the sham group and the experimental group were decreased (P lt;0.05, the expression of the latter two showed no significant difference (Pgt; 0.05). Conclusion ovariectomized mice lost control after estrogen, ZFP580 gene was down regulated, in ovariectomized mice given estrogen on a regular basis, ZFP580 expression levels returned to sham group, indicating that estrogen can increase ZFP580 gene expression. [Keywords:] ZFP580 gene, estrogen, hyperlipidemia, atherosclerosis, C57BL / 6 mice [Abstract] Objective To investigate the effect of estrogen on ZFP580 gene expression in the aortic tissue of C57BL / 6 mice. Methods The C57BL / 6 mice were made hyperlipidemia model and divided into three groups: sham ovariectomized, ovariectomized and ovariectomized with estrogen groups. Then the ZFP580 gene expressions were analyzed by semi quantitative reverse transcriptase polymerase chain reaction (RT PCR in the three groups. Results Results The ratio of ZFP580 gene and actin gene was significantly lower in the ovariectomized group than that of sham ovariectomized and ovariectomized with estrogen the other two groups (P lt;0.05). While the ra