EGFR monoclonal antibody C225 in Fe3O4-Ag coupled composite nanoparticles and identification of antibody activity in nasopharyngeal carcinoma cell proliferation.docVIP

 PAGE \* MERGEFORMAT 11 EGFR monoclonal antibody C225 in Fe3O4/Ag coupled composite nanoparticles and identification of antibody activity in nasopharyngeal carcinoma cell proliferation Of: Zhao Di, Sun Xinchen, Ma Jun, Tong Jinlong, Xu wisdom, Zhang Wei, Xiao Weihua [Abstract] Objective: To study the multi-functional nano-materials Fe3O4/Ag/C225 in vitro stability, targeting, and anti-tumor effect. Methods: The release composite nanoparticles Fe3O4/Ag level; by the initial detection of MTT colorimetric Fe3O4/Ag/C225 suppression of NPC tumor cell growth; by ELISA assay on the composite nanoparticles Fe3O4/Ag/C225 C225 stability. Results: The slow release composite nanoparticles Fe3O4/Ag/C225; coupling In the composite nanoparticles Fe3O4/Ag/C225 maintained on the C225 antibody itself is about 82% of the activity; composite nanoparticles significantly inhibited tumor cells of nasopharyngeal carcinoma. Conclusion: Fe3O4/Ag/C225 in vitro stability, coupled C225 The composite nanoparticles preserved antibody activity, inhibition of tumor cells of nasopharyngeal carcinoma .Fe3O4/Ag/C225 molecular imaging is expected to develop into a multi tracer IMRT. [Keywords:] Fe3O4/Ag/C225; nm; NPC [Abstract] Objective: To study the stability, targeting and antitumor effect of multi functional nanoparticles Fe3O4/Ag/C225 in vitro. Methods: The release ratio of Fe3O4/Ag/C225 was tested. Inhibitory effect of Fe3O4/Ag/C225 on nasopharyngeal carcinoma tumor cell growth was detected by MTT assay. The level of preserved antibody activity in the composite nanoparticles coupled C225 was detected by ELISA. Results: Composite nanoparticles released slowly. Experiments showed that composite nanoparticles inhibited tumor cells of nasopharyngeal carcinoma obviously.The C225 antibody coupling the composite nanoparticles Fe3O4/Ag was maintained well. Conclusion: The composite nanoparticles shows the activity inhibition of nasopharyngeal carcinoma in vitro.The composite nanoparticle