Elow molecular weight heparin on selectin-mediated HL60 cells and vascular endothelial cells adhesion.docVIP

 PAGE \* MERGEFORMAT 13 Elow molecular weight heparin on selectin-mediated HL60 cells and vascular endothelial cells adhesion Study: De-Cai Fu Xiao-Li Zhang Song Baohui [Abstract] Objective To study the low molecular weight heparin (LMWH on E selectin-mediated HL 60 cells and vascular endothelial cell adhesion. Methods isolated by primary culture of human umbilical vein endothelial cells (HUVEC and the establishment of in vitro capillary flow laminar flow system, the use of LMWH observed in the system under dynamic conditions in vitro with TNF @ HL 60 cell adhesion to HUVEC induced effects, measured by fluorescence plate reading of LMWH on the expression of E selectin. Results TNF @ treatment HUVEC 5 h can significantly increase the HL 60 cells and HUVEC adhesion under dynamic conditions, and could promote HUVEC surface expression of E selectin .0.1,1 mg / ml in vitro adhesion of HL 60 cells was significantly inhibited, 0.01,0.1,1 mg / ml of umbilical vein endothelial E selectin expression was inhibited. Conclusions LMWH significantly inhibited in vitro HL 60 cells under dynamic conditions with human umbilical vein endothelial cell adhesion and reduced vascular endothelial cell E selectin expression role. This result LMWH as an anti-inflammatory drugs, especially for the selectin as a target of drugs used clinically to provide an important experimental basis. [Keywords:] The low molecular weight heparin, cell adhesion, human umbilical vein endothelial cells, E selectin Cell adhesion is an important physiological phenomenon the body, and leukocyte adhesion to vascular endothelial cells and the maintenance of normal activities of the body mediated inflammation plays an important role (1 to 3.) Leukocyte adhesion under flow condition is a complex multi-step cascade process, several families of adhesion molecules involved in this process. Among them, the selectin family of adhesion mainly in the initial stages of white blood cells play a role. As the im