Europe and Asia Inula flavonoids on rat focal cerebral ischemiareperfusion injury in rats.docVIP

 PAGE \* MERGEFORMAT 8 Europe and Asia Inula flavonoids on rat focal cerebral ischemiareperfusion injury in rats [Abstract] Objective To observe the Europe and Asia Inula flavonoids (total flavones extracted from Inula britannica L, TFIB) on focal cerebral ischemia - reperfusion injury in brain tissue and to further explore its mechanism. Methods The middle cerebral artery embolization in rats produced focal cerebral ischemia-reperfusion injury model, ischemia 2 h, after 22 h reperfusion to hydrochloric acid 2, 3, 5  triphenyl tetrazolium (TTC) staining Determination of infarct size, colorimetry brain tissue superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, using sub-France ‘5 ‘for neurological symptom score. Results TFIB (25,50,100 mg * kg-1, ip) can significantly improve the cerebral ischemia - reperfusion of rat neurological symptoms, significantly reduced infarct area and the whole-brain area ratio, increased SOD activity in ischemic brain tissue reduce the MDA content. Conclusion TFIB on ischemia - reperfusion injury in the brain has significant protective effect, its mechanism may be related to TFIB antioxidant effects. [Keywords:] Europe and Asia Inula flavonoids; cerebral ischemia  reperfusion; anti-oxidation Abstract: ObjectiveTo investigate the protective effects of total flavones extracted from Inula britannica L. (TFIB) on cerebral ischemia-reperfusion injury in rats and its mechanism.MethodsThe model of focal reperfusion injury after cerebral ischemia in rats was made by middle cerebral artery occlusion. For 2h, followed by 22 h reperfusion. The infarction area of brain was assessed in brain slices stained with 2% solution of 2, 3, 5-triphenyl tetrazolium chloride. Spectrophotometric assay was used to determine the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in brain tissue. Neurological deficit was evaluated by using a 5-point neurological function scoring system.ResultsTFIB (25, 50,