Experimental rat model of febrile silt preliminary study.docVIP

 PAGE \* MERGEFORMAT 18 Experimental rat model of febrile silt preliminary study Author: Xu, Jiang Bao-ping, Guo Wei-feng, Wu Min-hua, Tai-Hui Fang 【Abstract】 Objective To investigate an animal model to establish thermal deposition method. Method of interval 24 h, from the rat tail vein injection of Escherichia coli endotoxin (LPS), given observed in rats at different times after endotoxin body temperature, blood coagulation time, blood viscosity and arterio-venous thrombosis and other indicators of changes in short-circuit. The results compared with the normal control group, 50 μg / kg in rats endotoxin can cause fever, increased blood viscosity, coagulation system activation and thrombosis, and the indicators show changes in phase coherence, the best time point is the 2nd to endotoxin after 2 h. Conclusion 100 μg / kg endotoxin injection interval of 24 h, can form a deposition heat syndrome in experimental rats. Keywords: silt heat syndrome; blood rheology; platelet aggregation; thrombosis Abstract: ObjectiveTo explore a method of establishing an experimental animal model mixed stasis-heat syndrome in rat in vivo. MethodsThe body temperature and parameters of hemorrheology were monitored at different time after the lipopolysaccharide (LPS) was treated by infusion vein through vena caudalis twice every 24 hours in rats. ResultsThe body temperature, viscosity of whole blood, viscosity of plasma, the activity of coagulation-fibrinolysis system were significantly increased compared with control group, and the optimal time is the 2nd hour after the last treating with LPS.ConclusionRats treated with LPS twice every 24h with total doses of 100μg/kg result in symptom of “stasis” and “heat” of mixed statsis-heat syndrome. Keywords:: Mixed stasis-heat syndrome; Hemorrheology; Platelet aggregation; Thrombus Professor Zhou Zhongying the country were the old traditional Chinese medicine on the basis of understanding of the ancien