Experimental Study on Effects of catecholamine.docVIP

 PAGE \* MERGEFORMAT 12 Experimental Study on Effects of catecholamine 【Key Words】 summer knee oral liquid; spontaneously hypertensive; catecholamine In recent years, studies have shown that, in the pathogenesis of hypertension is often demonstrated the nervous system, endocrine system posed by the complex network of dysfunction, during which the information linked mainly through neurotransmitters, endocrine hormones and cytokines to complete. Catecholamine (CA) is an important regulator of cardiovascular events and an important neurotransmitter regulating hormones in the body can directly act on the blood vessels involved in the heart of the physiological and pathological processes, and its plasma concentration and the incidence of hypertension closely. Is by observing the SHR rat serum catecholamine levels in the summer of oral antihypertensive mechanism of the knee. An experimental material 1.1 Experimental Animal 12-week-old male SHR 40, with the same-week-old male Wistar  Kyoto rats (WKY) 10, with body weight (230 ± 20) g (by Beijing Tong Lihua Victoria Company). 1.2 Drugs and reagents summer knee Liquid (from Changchun University of Chinese Medicine First Affiliated Hospital of preparation room provided batch number: 030,108); captopril tablets (Fahrenheit Shanghai Pharmaceutical Co., Ltd., batch number: 060,601); E Beisha Tan tablets (revised Pharmaceutical Group Co., Ltd., batch number: 060,501); Catecholamine ELISA detection kit (Shanghai Matsa Bio-Engineering Co., Ltd.). 1.3 main instruments BP  6 non-invasive animals, blood pressure measurement system (Chengdu Thai Union Technology Co., Ltd.); MK  3 automatic ELISA analyzer (Shanghai PHILIP associated companies); Jingli LDZ5  2 centrifuge (Beijing Medical Centrifuge Factory) ; JIN Yi MM  2 micro-oscillator (China Jintan Medical Instrument Factory). 2 Experimental Methods 2.1 The experimental group before the experiment each r