Fang Kai phrenic cell proliferation in human gastric cancer MGC exercise capacity and secretion of MMP-2 MMP-9 enzyme activity.docVIP

 PAGE \* MERGEFORMAT 13 Fang Kai phrenic cell proliferation in human gastric cancer MGC exercise capacity and secretion of MMP-2 MMP-9 enzyme activity Of: Li Jing, Li, Liu Yaxian, Lu Fu River, Fu Xiaomei [Abstract] Objective To observe the start side of the diaphragm cell proliferation in human gastric cancer MGC, athletic ability and secretion of matrix metalloproteinase (MMP) -2, MMP-9 activity of. Methods Cultured human gastric cancer MGC cells were divided into Kai phrenic side of small, medium and large dose group and 5 - fluorouracil (5-FU) group and blank control group, a total of 5 groups. Kai diaphragm side of small, medium and high dose groups with different concentrations of the medicine, the final concentration of 35,70 , 140 mg / mL; 5-FU group was added to a final concentration of 5-FU was 50? g / mL, blank control group to join PBS. were detected by MTT proliferation of tumor cells, tumor cells detected by agar drop exercise capacity, Gelatin zymography of tumor cells to secrete MMP-2, MMP-9 activity. Results Kai diaphragm side MGC cells significantly inhibited proliferation and its inhibition with the drug concentration is more obvious. Kai diaphragm side of each dose Group ,5-Fu group exercise capacity was significantly weaker than the control group, movement is slow, the difference was statistically significant (P lt;0.05). Kai diaphragm side of each dose group cells MMP-2, MMP-9 activity was significantly higher than the control group decreased (P lt;0.05), in which MMP-2 activity was more significant (P lt;0.01). Conclusion MGC Kai diaphragm before tumor cells by inhibiting the proliferation, athletic ability and MMP-2, MMP-9 activity to inhibition of metastasis. [Keywords:] Kai diaphragm side; cell culture; cell proliferation; cell movement; matrix metalloproteinase -2; matrix metalloproteinase -9 Abstract: Objective To observe the inhibitory action of Qigefang on cell proliferation, movement, secreting MMP-2 and MMP-9 of MGC ga