Fluorescence Determination of rhubarb in the content of free anthraquinones.docVIP

 PAGE \* MERGEFORMAT 13 Fluorescence Determination of rhubarb in the content of free anthraquinones Author: Wang Peiqi, Cheng-wei, GUO Xing-jie, FAN Hui-yu 【Abstract】 Objective fluorescence spectrophotometry, was established rhubarb anthraquinones in the free determination method. Method using acetone as the solvent, the λex = 460 nm and λem = 540 nm fluorescence intensity was measured. The results measured free anthraquinone content in 0.25 ~ 3.0 μg / ml showed good linearity within the relationship, linear regression equation is F = 122.81C 46.224 (r = 0.999 6), the minimum detection limit was 0.25 μg / ml, showed that The method of high sensitivity, good reproducibility, and easy to use. Conclusion The study for measuring the active ingredient in free rhubarb anthraquinones provides an effective and reliable analysis method. Keywords: rhubarb; free anthraquinone; fluorescence spectrophotometry Fluorescence Spectrophotometry for Free Anthraquinones in Rheum emodi Abstract: ObjectiveWith the fluorescence spectrophotometry, to build up free anthraquinones in Rheum emodi method of Assay.MethodsTake acetone as solvent agent, with the λex = 460 nm and λem = 540 nm determination fluorescence strength. ResultsFree anthraquinone contents were in 0.25 ~ 3.0 of μg / ml with good linear relationship. The linear equation F = 122.81C 46.224 (r = 0.999 6), the lowest detectability was 0.25 μg / ml. The method was sensitive and reproducible.ConclusionThis research for measuring live composition of free anthraquinone in Rheum emodi Wall provides a kind of effectively dependable analytical method. Keywords:: Rheum emodi; Free anthraquinone; Fluorescence spectrophotometry Active ingredient content is an important indicator of the quality evaluation of medicinal herbs, rhubarb is now reported detection of free anthraquinones are mainly colorimetry, thin-layer chromatography (TLC) method, high performance liquid chromatography (HP