Focal cerebral ischemia model in rats Preparation and Experimental Study of the brain.docVIP

 PAGE \* MERGEFORMAT 17 Focal cerebral ischemia model in rats Preparation and Experimental Study of the brain [Abstract] Objective: To study the middle cerebral artery in Wistar rat model of cerebral ischemia animal preparation methods and different specifications perfusion comparative study of brain was removed, summarize key aspects and considerations. Methods: ligation of common carotid artery (CCA), carotid artery (ECA), the pterygopalatine artery was not ligated, the thickness of the fish with the appropriate line of the blind from the CCA into the internal carotid artery (ICA) until the middle cerebral artery occlusion, pull reperfusion; the stipulated time of 4% paraformaldehyde buffer Heart brain was removed. Results: The success rate modeling, modeling a typical clinical manifestations of animals; non-perfused the brain, brain structure-retention is poor; short-term perfusion fixation of brain cortex and hippocampus to retain good structure, but deep white matter and nuclei structure preserving difference; long-term perfusion fixed retention structure is good, but time-consuming, stationary use large, deep white matter of brain and effect structure of nuclei. Conclusion: Preparation of this model easy to operate and detailed considerations, credibility high; of the cortex and hippocampus choose short-term ischemia reperfusion can still meet the needs of the fixed brain was removed, for studies of deep white matter and nuclei structure still need long-term perfusion fixation of brain. [Keywords:] ischemia-reperfusion injury; animal model; brain Abstract Objective: To explore a modified surgical approach to produce rat ischemia-reperfusion injury model and to study the perfusing fixative solution method of drawing the material, moreover, to summarize key points and critical items for attentions. Methods: After exposure and ligation of the common carotid artery (CCA) and the external carotid artery (ECA), the pterygopalatine artery were opened, t