HL60-ADR emodin inhibited cell proliferation and induce resistance to apoptosis.docVIP

 PAGE \* MERGEFORMAT 2 HL60/ADR emodin inhibited cell proliferation and induce resistance to apoptosis Author: Chen Ying-yu, ZHENG He-Yong, Hu Da, ZHENG Zhi-hong, Zheng Jing, even Xiaolan Lu Lianhuang [Abstract] This study traditional Chinese medicine emodin (emodin)-resistant human leukemia cell line HL  60/ADR proliferation, apoptosis and its corresponding possible mechanism. MTT method using cell growth curve mapping; colony formation was observed by emodin on HL  60/ADR colony forming impact; cell cycle analysis, mitochondrial transmembrane potential detection, Caspase  3 activity detection, Annexin V FITC / PI method, TdT Enzyme-mediated in situ nick end labeling (TUNEL) analysis of apoptotic cells; RT  PCR and Western blot to detect the role of emodin different time points after bcl  2, c  myc mRNA and Bcl  2, c  Myc, Caspase  3 precursor protein expression level changes. The results showed that emodin on HL  60/ADR significantly inhibit cell proliferation, was time-and concentration-dependent. Lower concentrations of emodin can inhibit HL  60/ADR colony-forming cells, IC50 value of 5.79 μ mol / L. Cell cycle analysis showed that compared with the control group, 40,80 μ mol / L concentration group, cells were blocked in the G0/G1 phase ratio was significantly higher (p